Day 1 :
- Track 1: Advances in Sample Preparation Techniques
Track 2: Membrane Processes
Track 3: High-Impact Application Fields
Session Introduction
Yongjae Lee
Food Protein R&D Center at Texas A&M University, USA
Title: Membrane & Other Separation Technologies and Their Application to Food Technology
Biography:
Dr. Yongjae Lee is a Head of Separation Science at Food Protein R&D Center at Texas A&M University, USA. In this occupation he directly leads the Separation group in the lab and in the pilot plant research on the separation technologies of food, dairy, feed, beverage, specialty ingredients, biotechnology and biofuel, and oil & gas. He also hosts short courses in the area of membrane, and other traditional and advanced separations techniques. He has more than 12 years of industrial experience in the field of microbiology, chemical engineering, and agricultural engineering. He has published several papers in journals and has presented numerous invited lectures in various national and international meeting. Dr. Lee holds a Ph.D. in Animals & Veterinary Science from the Clemson University, Clemson, South Carolina, USA and a Master’s degree in Food Science & Technology from the Louisiana State University, Baton Rouge, Louisiana, USA.
Abstract:
In food industry, separation technology is broadly used to separate, isolate, and purify a particular component from a mixture. These are essential in the food manufacturing process. A numerous separation technologies are available such as using membrane, centrifuge, decanter, spray dryer, freeze dryer, and extractor. Especially, membrane technology has been broadly used in the food industry since 5-6 decades due to their processing at a lower temperature and less energy-intensive. Typically, Microfiltration (MF), Ultrafiltration (UF), Nanofiltration (NF), and Reverse Osmosis (RO) are commonly used pressure-driven membrane separation in the food processing. These types differ in membrane characteristics, pore size, and operating pressure. The major membrane applications in the food are dairy and beverage industries. Microfiltration widely used to remove bacteria and spore, fat, and casein from skim milk. Ultrafiltration is used to remove lactose, and increase or decrease the protein content. Nanofiltration is used to concentrate and partially demineralize liquid products. Reverse osmosis is mainly used to remove water from a mixture which increase the product concentration with dehydration. Despite the many advantages of membrane technology, it has the key disadvantage which is the fouling of the membrane. It causes a reduction in flux rate and thus decreasing productivity over time. Back flux and chemical cleaning process can be applied to minimize the fouling but not perfect. Advanced technology such as grafted coating, zeolite, and graphene can be applied to solve the fouling issues.
Catia Algieri
Institute on Membrane Technology ITM-CNR, Italy
Title: MFI Zeolite Membranes for Water Desalination
Biography:
Catia Algieri is researcher at the Institute on Membrane Technology (ITM-CNR, ITALY) from 2001. Her research activity is focused on the preparation and characterization of organic and inorganic membranes for gas separations, water treatment and catalytic reactions. She has published many papers in reputable journals and she has presented numerous invited lectures in different international congresses. She is member of the Editorial Board in Journal of Crystallography and referee of several international Journals.
Abstract:
Zeolite membranes due to their crystalline structure and to their pore diameters close to the molecular size of different species have attracted the interest of many researchers in the separation processes field. They can be also used as membrane reactors owing to their high thermal and chemical resistance. Considering all these properties, water separation from organic solvents and water treatmentare other possible applications of these membranes. For example, about the ion removal from water, very interested in desalination process is the employ of MFI membranes, because their pore size (about 5.5 Å) is lower than the major kinetic diameters of various hydrated ions.In this work MFI zeolite membranes were synthesized on ï¡-Al2O3 tubular supports. In particular, the membranes were prepared by a secondary growth method using the cross-flow seeding procedure. Subsequently, these membranes were characterized by means of single gas and pure water permeation tests and then used in vacuum membrane distillation to investigate their potential application in water desalination.The membrane performance in distillation process was investigated using both distilled water and salt solutions with different NaCl concentration (0.2, 0.6 and 1.2 M) and using two different operating conditions. In the first case the membranes were tested in a continuous way without washing treatment. In the second one the system was washed at the end of each experiment.The results evidenced high and constant fluxes and salt rejection values higher than 98% for all the concentration considered.
Kirk J. Ziegler
University of Florida, USA
Title: The Role of Surfactant Structure in the Separation of Single Walled Carbon Nanotubes (SWCNTs)
Biography:
Professor Ziegler received his PhD in 2001 from the University of Texas at Austin. After a postdoctoral position in Prof. Smalley’s laboratory, he joined the Chemical Engineering Department at the University of Florida in 2005. His research group focuses on understanding the role of interfaces in one-dimensional nanostructures, such as single wall carbon nanotubes (SWCNTs) and vertical arrays of nanowires. His work on SWCNTs has focused on understanding the effect of surfactant-nanotube interactions on dispersion and separation processes. The ability to control these interfaces allow for efficient separation of SWCNTs and their integration into composite structures.
Abstract:
Single-chirality single walled carbon nanotubes (SWCNTs) have unique optoelectronic properties that can be utilized in specific applications, such as photovoltaics and biosensor. However, due to the large variety of SWCNTs and the difficulty of separation, the application of chiral SWCNTs is limited. The post-synthesis separation of SWCNTs has been studied with great interest in the past decade. The most promising techniques include the selective adsorption of SWCNTs onto hydrogel stationary phases and the aqueous two-phase extraction. The surfactant structure surrounding single walled carbon nanotubes (SWCNTs) plays an important role in their separation by nearly any method. However, characterizing the structure of these molecular layers remains difficult. The structure of the surfactant or other molecules around the SWCNTs could also have important implications in toxicology and drug delivery. Using our understanding of the surfactant structure surrounding SWCNTs, we developed high-fidelity separations of nanotubes by both selective adsorption and two-phase extraction. Finally, we will describe how these mono-chiral suspensions can be used in various applications.
Prof. (Neal) Tai-Shung Chung
National University of Singapore, Singapore
Title: Advanced separation technologies based on polymeric membranes for clean water and clean energy
Biography:
Prof. Chung had worked for US industries for 15 years before joining NUS in 1995. He is a Subject Editor of Chemical Engineering Research and Design and editorial board members of 15 journals including J. Membrane Science, AIChE J., I & EC Research, Separation and Purification Reviews, and others. He was an inventor of Hyflux Kristal™ 600 ultrafiltration membranes. He received IES (Institution of Engineers, Singapore) Prestigious Engineering Achievement Award, Hyflux-SNIC (Singapore National Institute of Chemistry) Award in Environmental Chemistry in 2010, and Research Leadership Award at NUS in 2011. He became a Fellow in the Academy of Engineering Singapore in 2012. He received the 2014 Underwood Medal for Exceptional Research in Separations from IChemE (Institute of Chemical Engineers, UK).
Abstract:
Clean water, clean energy, global warming and affordable healthcare are four major concerns globally resulting from clean water shortages, high fluctuations of oil prices, climate changes and high costs of healthcare. Clean water and public health are also highly related, while energy is essential for sustainable prosperity. Among many potential solutions, advances in membrane technology are one of the most direct, effective and feasible approaches to solve these sophisticated issues. Membrane technology is a fully integrated science and engineering which consists of materials science and engineering, chemistry and chemical engineering, separation and purification phenomena, environmental science and sustainability, statistical mechanics-based molecular simulation, process and product design. In this presentation, we will introduce our efforts on advanced membrane separation technologies for clean water (nano-filtration, membrane distillation, forward osmosis) and clean energy (osmotic power generation, natural gas, hydrogen, and biofuel). Technology breakthroughs in each area will be highlighted.
Hiroshi Uyama
Osaka University, Graduate School of Engineering, Japan
Title: Fabrication of Porous Monolithic Materials of Reactive Polymers for Protein Purification
Biography:
Hiroshi Uyama received his B.S. (1985) and M.S. (1987) from Kyoto University. In 1988, he joined the Department of Applied Chemistry, Tohoku University, as Research Associate and obtained Ph.D. He moved to the Department of Materials Chemistry, Kyoto University in 1997. In 2004, he was appointed as a full professor at the Department of Materials Chemistry, Osaka University. He has published over 270 original papers, 170 book chapters and reviews, and 140 patens.
Abstract:
Along with the rapid development of diagnosis and proteomic science, the requirement for the purification of biomolecules like proteins, enzymes and nucleic acids becomes extremely urgent. Protein A chromatography and immobilized metal ion affinity chromatography are regarded as the most efficient and promising methods for the protein purification. For these two techniques, the solid support is the crucial part for the protein purification efficiency and specificity. Polymer-based monoliths with tunable bulk and surface properties have attracted considerable attention due to their unique open-cellular three-dimensional porous structure. Recently, we have developed fabrication of such monolithic materials by phase separation of polymer solutions. The present talk deals with functional monoliths based on reactive polymers such as cellulose and poly(vinyl alcohol-co-ethylene) for protein purification. Protein A was introduced on the monolith of these polymers via suitable chemical activation. For an application of immobilized metal ion affinity chromatography, ethylenediaminetetra acetic acid was introduced into these monoliths and nickel ions were located subsequently. IgG and His-tagged proteins were immobilized on these activated monoliths. The protein purification amount and efficiency were evaluated.
Simona Liguori
Stanford University, Energy Resources Engineering Dept., USA
Title: Inorganic Metallic Membranes for Gas Separation
Biography:
Simona Liguori is a Physical Science Research Associate at Stanford University. She earned her MS in Chemical Engineering and PhD in Environmental Science and Sustainability. Se has over 8 years of research experience in membrane and membrane reactor technology related to the highly pure hydrogen production from bio-fuels reactions via membrane reactors and CO2 separation. She published more than 20 peer-reviewed articles; more than 10 chapters on international books on membrane science. Referee for several international scientific journals.
Abstract:
Currently, membrane technology for gas separation covers an important role in reducing the environmental impact and costs of industrial processes. In particular, it offers a number of benefits over other gas separation technologies as higher energy efficiencies, greater operational flexibility as well as simplicity of operation and maintenance. At the moment, polymeric membranes are the most widely used for gas separation. Nevertheless, some issues still remain regarding the scalability and reliability of the polymeric materials under real operational conditions where the temperature is often too high for polymer stability. Metallic membranes, by contrast, usually require high temperature for operation and may be more beneficial in saving energy under high temperature conditions. In particular, inorganic H2 selective membranes have gained a great attention in the field of the hydrogen economy development. Due to the characteristics of hydrogen perm-selectivity with respect to all other gases, palladium and its alloys play the role of dominant material in this field. Metallic membranes could be also used for N2 removal from natural gas or from coalfired flue gases located nearby the boiler exit, which may result in increased concentrations of CO2 and pollutants with a significantly reduced gas volume in the downstream, allowing for traditional emissions controls to perform more efficiently and, consequently, lowering the overall energy consumption and capital and operating costs. However, some issues need to be addressed, such as the development of thin membranes with long-term thermal and mechanical stability and resistant to the surface poisoning.
Mohammed W. Hakami
Yanbu Industrial College, Saudi Arabia
Title: Discharge times of 3.0 micron superfine powders from a circulating fluidized bed of a binary mixture
Biography:
Dr. Mohammed Hakami has completed his PhD in Chemical Engineering from Swansea University, United Kingdom on October 2013 in the field of water treatment using membrane. His work is in membrane technology. He is interested in separation techniques and he teaches separation process courses at Yanbu Industrial College. E-mail: mohhakus97@gmail.com
Abstract:
The high gas velocities in Circulating Fluidized Beds (CFBs) often result in fine powders becoming discharged and lost in the fluid going out at the cyclone upside stream. Thus, residence times of these fines in the bed will drastically fall together with the overall performance of the whole fluidizing system. The discharge time, TD, represents the time needed for all the fines to be discharged out of the bed. Therefore, the loading of superfine in the bed decreases. The discharge times of micron-size superfines from a semi-batch CFB were investigated using a binary mixture of superfine aluminium hydroxide powders (3.0 μm mean size) and coarse FCC particles (66 μm mean size). The discharge times of superfines are believed to be affected not only by gas humidity, but also by the water content of the bed particles. The effects of the equilibrium water content of FCC particles on the discharge times of superfines were investigated under different gas velocities and starting loadings of superfines of 3 and 5 wt.%. At a certain gas velocity, the discharge times decreased sharply using FCC particles of higher moisture contents as 0.054–0.067 wt H2O/wtdry FCC, irrespective of the loading of superfines. Dry FCC particles and FCC particles of lower moisture contents as 0.038 wt H2O/wtdry FCC, were found not to be appropriate for CFB as extremely large discharge times of superfines were obtained at higher loadings of superfines. High gas relative humidity at 85% could not decrease the discharge times of superfines in the presence of totally dry bed particles.
Z. Mansooria
Amirkabir University of Technology, Iran
Title: Wall Roughness Effect on Particle Separation Rate of The Turbulent Gas-Solid flow in inclined pipes
Biography:
Zohreh Mansoori completed her PhD in Mechanical Engineering from Amirkabir University of Technology ( Polythechnic), Tehran, Iran. She is associate professor and the head of Energy Research center in Amirkabir University. She has published more than 35 papers in reputed journals and has been serving as an editorial board member of repute.
Abstract:
The effect of wall surface roughness on the separation of solid particles in the turbulent gas-solid flow in the pipes with different inclination angles is studied. Inclined pipes are used in many industrial applications such as pneumatic transfer lines, heat exchangers and gas transport pipelines. The numerical model for 3D pipe considering four- way interaction is used to solve turbulence intensities for dynamic field. Interaction of the particles with rough wall is modeled introducing available stochastic wall roughness models for the dispersed phase to the computational program. It is assumed that the particles collide the wall surface would be omitted. It is found that changes in the particle dispersion and particle concentration results in the separation rate change in the different regions of the pipe. Also, the wall roughness could affect the particle- wall collision and the solid particle separation rate.
Ping Hu
China University of Petroleum, China
Title: High-efficiently simultaneous oxidation of organo-arsenic and immobilization of arsenic in Fenton enhanced plasma system
Biography:
Dr. Ping Hu currently working as professor at China University of Petroleum, Tsingtao, Shandong Sheng, China. He has published several publications as the first author or corresponding author in journals including Dual enhancement-inhibition roles of polycarboxylates in Cr(VI) reduction and organic pollutant oxidation in electrical plasma system
Abstract:
Roxarsone (ROX) is heavily utilized in agricultural applications and poses a risk to the environment. The applicability of glow discharge plasma (GDP) for simultaneous oxidation of organo-arsenic and immobilization of arsenic is unprecedentedly evaluated in this study. The results show that ROX can be effectively oxidized to inorganic arsenic and this performance is evidently dependent on energy input. Adding Fe(II) significantly enhances the oxidation of ROX mainly because of the additional production of •OH via Fenton reaction in GDP, accompanied with which the generated arsenic can be immobilized in one process. Arsenic immobilization can be favorably obtained at pH 4.0-6.0 and Fe(II) concentration from 500 to 1000 mM. Based on the mineral compositions and the analysis (XRD/FTIR/XPS) of precipitate, a mechanism can be proposed that the oxidation of Fe(II) by H2O2 generated in situ in GDP significantly accelerates ROX transformation to the ionic As(V), which can immediately co-precipitate with Fe(III) ions or be adsorbed on the ferric oxyhydroxides, forming amorphous ferric arsenate-bearing ferric oxyhydroxides. Consequently, the Fenton enhanced GDP process exhibits as an economical and versatile strategy for organo-arsenic oxidation and arsenic immobilization, holding a premise for the remediation of organic arsenic wastewater.
Marek Trojanowicz
Institute of Nuclear |Chemistry and Technology, Poland
Title: Biosensors as Detectors in High Performance Separation Techniques
Biography:
Marek Trojanowicz has completed his M.Sc., Ph.D. and D.Sci. degrees in Department of Chemistry, University of Warsaw. Currently he is full professor in Institute of Nuclear Chemistry and Technology in Warsaw, Poland. He is author of 300 scientific papers, 2 monographic books and editor of 1 book in the field of flow analysis and automation of analytical measurements. His main scientific interests include design of electrochemical sensors and biosensors, flow analysis, liquid chromatography and capillary electrophoresis, application of ionizing radiation for water and waste treatment and application of chemical analysis in archaeometry.
Abstract:
Biosensors are analytical devices incorporating a biological material e.g. tissue, microorganisms organelles, cell receptors, enzymes, antibodies, nucleic acids etc. intimately associated with or integrated with a physicochemical transducers using optical, electrochemical, thermometric, or piezoelectric properties. They have a solid place in contemporary analytical chemistry, which is evident from their very strong position on the market of analytical instruments, mass production of many of them, uncounted applications in various fields, and a very large research potential directed for this field in academia and industry. This is field of modern science and technology, which immediately adapts current achievements and discoveries in various branches of science, electronics, material science and micromechanics. Hyphenation of biosensing with separation methods can be realized practically with all types of biosensors, and many different electro migration and chromatographic methods. This can be a very efficient way for improvement of selectivity of biosensing, and with suitable design of the flow-through cell and whole measuring setup with on-line sample processing, it allows also improving sensitivity of whole analytical procedure. Another especially valuable application of this concept is design of multi-analyte detection systems with application of enzymes that catalyze reactions of a group of products, or are inhibited by a group of similar compounds. Especially interesting examples of such systems include, for instance electro-antennographic detection in liquid chromatography, olfactory detection in gas chromatography, or single-cell based detection in capillary electrophoresis.
Sena Caglar
Istanbul University, Turkey
Title: Direct analysis of drugs in biological fluids by on-line chromatography
Biography:
Sena Caglar is a Research Assistant at the Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry. She had PhD and MSc degrees at the same department and studied Chemical Engineering. She conducted post-doctoral research at Medical Center of Munich University, Institute of Laboratory Medicine, Laboratory of Bio-Separation. She has researches on drug analysis in biological fluids by liquid chromatography mass spectrometry, on-line solid phase extraction coupled liquid chromatography, multidimensional chromatography and publications in drug analysis in pharmaceutical formulations and biological fluids by high performance liquid chromatography, spectrometry, degradation, pharmacokinetic and validation studies.
Abstract:
In pharmaceutical, bioanalytical and biomedical analysis of drugs and metabolites in biological fluids is essential for bioequivalence/bioavailability, therapeutic drug monitoring and drug abuse studies. An optimal and effective sample preparation method plays the most important role since the depletion of the matrix in biological fluids is the biggest issue for a trouble-free analysis. It is impossible to inject the biofluid directly to the chromatographic system with traditional methods but this challenge was overcome by the on-line methods. Combination of solid phase extraction (SPE) with high performance liquid chromatography allows direct analysis of small molecules (i.e., drugs) in biofluids. The method, in summary, depends on connection of the SPE column, coated with various packing materials, directly to the analytical column (where the analytes are separated) through a switchingvalve and injection of the sample to the system. Following the injection, i) the matrix components are depleted and ii) the analytes are separated in the column. Thus, sample preparation step from the biological fluid is completely eliminated and the sample can be directly injected to the system resulting with high reproducibility in the analyses.
- Track 6: Advances in ChromatographyTrack 7: Advances in Mass Spectrometry
Session Introduction
Pao-Chi Liao
National Cheng Kung University, Taiwan
Title: Metabolomics approaches for bio-marker discovery for toxicant exposure of di-isononyl phthalates (DINPs) using liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Biography:
Pao-Chi Liao completed his PhD in Analytical Chemistry from Michigan State University (MSU) in 1995 before doing Postdoctoral research in the Department of Biochemistry at MSU. He joined as the Faculty at Department of Environmental and Occupational Health, National Cheng-Kung University, Taiwan in 1997, where he was promoted to full Professor in 2006, and named Distinguished Professor in 2011. His research interests and fields of specialty include analytical chemistry, mass spectrometry, proteomics, biomarker discovery, cancer biomarkers, lung cancer metastasis, and environmental and occupational health.
Abstract:
Di-Isononyl Phthalate (DINPs) is widely used as plasticizers and has effects on reproductive system. Three metabolomics approaches were used to explore DINP exposure markers in an LTQ/Orbitrap HRMS dataset obtained from liver enzyme incubation. The data processing techniques included Signal Mining Algorithm with Isotope Tracing (SMAIT), Mass Defect Filtering (MDF), and web-based XCMS. Fourteen metabolites were validated as DINP exposure markers using a rat model. Among the 14 exposure-related DINP metabolite signals, 8 have not been reported in the literature. The metabolomics platform can efficiently and systematically filter probable metabolite signals from a complex LC-HRMS dataset for toxic exposure marker discovery.
Vlad Orlovsky
SIELC Technologies, USA
Title: Evolution of mixed-mode from “mixing†silica gels to core-shell mixed-mode technology
Biography:
Vlad Orlovsky graduated with MSc in Organic Synthesis from Ufa Petrochemical University in 1987. In 1992, he moved to United States to continue developing his skills in organic chemistry. He joined Pfizer in 1993 as a Research Chemist. After working at Pfizer, he co-founded SIELC Technologies with a goal to develop better separation technologies. In collaboration with his colleagues, he developed over 23 new commercial stationary phases. His continuing research and development in this field resulted in development of the first ever core-shell mixed-mode stationary phases. He has 5 patents and a dozen publications in scientific journals.
Abstract:
In recent years, mixed-mode chromatography emerged as a powerful separation technique with alternative selectivity. Evolution of this technology covering old “mixing” approach and single ligand design is being discussed with the emphasis on selectivity benefits and critical applications. Comparison of single, dual, tri-modal columns are offered and evaluated. The first line of core-shell mixed-mode stationary phases is presented. Numerous examples in pharmaceutical, environmental, food and agricultural sciences are presented.
Biography:
Professor Asif Zaidi completed his PhD in Department of Physics & Astronomy. University of Waterloo from University of Waterloo in the year 2010. His research interests focus on controlled synthesis, separation, assembly and property regulation of functional inorganic nanomaterials and carbon materials. He has published more than 7 publications as the first author or corresponding author in journals including Small molecules from the decomposition of interstellar carbons
Abstract:
Solid methane at 77K was irradiated with laser pulses of wavelength 800 nm and energy of 275 μJ. Laser pulse duration was 100 fs. This irradiation experiment shows formation of higher molecular weight pure carbon clusters Cn where n = 1, 2, 3, ---, and the formation of alkane molecules of higher molecular mass than the original target molecule. These molecules have the composition (CH4)n where n = 1, 2, 3-- The time of flight spectrum also shows the formation of polyynes and cumulenes as ablation products. The laser ablation of graphite has been ex-tensively studied because of graphite’s unique properties. It is well known that the laser ablation of graphite with nanosecond pulses produces pure carbon clusters and fullerenes such as C50, C60 and C70 as well as more complex species including nanotubes, depending on ablation conditions. It is then important to investigate the ablation of molecular systems containing C-H sigma bonds using femtosecond laser pulses in order to see the effect of hydrogenation of the target molecule. The use of femto-second, rather than nanosecond laser pulses eliminates heating of the target. Alkanes are the simplest fully hydrogenated C-H molecular systems that occur naturally and methane (CH4), containing only C-H sigma bonds, is the simplest member of this family. A study of the ablation of solid methane is then important in understanding ablation in more complicated alkane systems. Experiments were carried out using a 5×10-7 torr vacuum chamber attached to a time of flight (TOF) spectrometer. By flowing methane gas onto a cold finger made of copper plate in the vacuum chamber, a 0.5 mm-thick film of solid methane (a van der Waals solid with a density of 0.522 gm cm-3) was obtained on the cold finger. Five to eight successive laser pulses chopped from a 100-fs 1-KHz 800nm 300-μJ mode-locked Ti:sapphire laser system were focused on the solid methane by a 10-cm focusing lens. Each focused laser pulse has a peak intensity 8×1014 W/cm2. A laser plume was generated which was extended from the cold finger surface up to a few millimeters above it. Positively charged particles generated in the laser plume were accelerated by a 4kV linear accelerator toward a micro-channel plate (MCP) detector. Signals from the MCP detector were recorded by a scope and sent to the PC.
Gerard Rosse
Dart NeuroScience, San Diego, CA
Title: Advances and Applications of Supercritical Fluid Chromatohgraphy in Drug Discovery
Biography:
Dr. Rosse is currently Associate Director, Structure Guided Chemistry, at Dart Neuroscience and serves as Adjunct Associate Professor at Drexel University. Prior he functioned in leadership and scientific positions in medicinal chemistry with Cephalon, Sanofi and F. Hoffman-La Roche. During his industrial tenure, he led multidisciplinary teams and invented pre-clinical candidates for a wide range of therapeutic indications including CNS, Inflammation, Metabolism, Oncology, and Antibacterial agents. Dr. Rosse’s career is also characterized by the implementation of innovative chemical technology and analytical chemistry platforms that accelerated the drug discovery process and reduced costs. He received the Ph.D. degree in chemistry from the University of Basel in Switzerland and postdoctoral training at Stanford University, California.
Abstract:
Innovative technologies to make better compounds and make them faster have been implemented. The presentation will focus on the design of a highly automated facility with a multistep process for the rapid analysis and purification of small molecules using Supercritical Fluid Chromatography-Mass Spectrometry (SFC-MS). The recent introduction of a new generation of SFC-MS instruments is revolutionizing the field of chromatography. The instrumentation and the robotic infrastructure to support the production of ~10,000 compounds/month will be discussed. Finally the benefits of an enhanced SFC-MS technology platform to increase productivity and decrease costs will be discussed.
Xiaoming Sun
Beijing University of Chemical Technology, China
Title: Nanoseparation-from Methodology to Application
Biography:
Professor Xiaoming Sun completed his PhD from Tsinghua University and postdoctoral studies in Prof. Hongjie Dai’s group at Stanford University. he is currently the vice president of inorganic chemistry department in Beijing University of Chemical Technology. His research interests focus on controlled synthesis, separation, assembly and property regulation of functional inorganic nanomaterials and carbon materials. He has published more than 60 research papers as the first author or corresponding author in journals including J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater. and Chem. Eur. J.. They have been cited more than 3500 times in total.
Abstract:
Density gradient ultracentrifugarion (DGU) separation method was established to obtain monodispersed colloidal nanostructures. Such separation method was demonstrated as a versatile method for acquisition of monodispersed colloidal nanoparticles which are hard to be synthesized. This separation method was applicable to both aqueous (polar) and organic (non-polar) solvents systems; and NPs with different size, density and morphology can be separated. Separation objects involve nearly all kinds of materials including metal and metal oxides/sulfides, carbon materials, semiconductors, etc. Synthesis-structure-property relationships were observed on the separated NPs, which guided synthetic optimization. Besides separation, concentration and purification of NPs could be achieved at the same time when a water/oil interface was introduced into the separation system. By introducing a reaction zone or an assembly zone in the gradient, we can monitor the reaction and assembly of NPs since reaction time could be controlled and chemical environments could be changed extremely fast. In short, “Lab in a tube” method paved a way for the research on nanoparticle synthesis optimization, purification, assembly and surface reactions.
Solich Petr
Charles University, Czech Republic
Title: Trends in development of stationary phases in chromatography
Biography:
Prof. Petr Solich has completed his PhD. from Charles University, Faculty of Pharmacy, Hradec Kralove, Czech Republic. He is the head of Department of Analytical Chemistry as well as head of University Research Centre UNCE at Faculty of Pharmacy, Charles University. He has published more than 180 papers in impacted analytically oriented journals, with h-index 28 and has been also serving as an editorial board member of journal Talanta.
Abstract:
High performance liquid chromatography is one of the most progressive separation techniques which allows identification and quantification in one step and is frequently used in clinical research for analysis of biological samples in connection with modern sample preparation. Choice of the sample preparation technique is key step which influence sensitivity, robustness, solvents and sample consumptions etc. Connection of modern, fast and robust sample preparation procedures and modern trends in liquid chromatography are useful base for clinical research. A huge expansion of new stationary phases was registered during last couple of years. Several different technologies with different characteristics were introduced into the market, among them Core shell technology using porous shell and solid core particles. These columns can be used in common HPLC instruments as well as in UHPLC systems. This technology promises to increase of resolution and maximizes throughput, and result in solvent saving and easier method transfer. The recently introduced HR monolithic technology is based on a unique sorbent material allowing good quality of separations in a minimal time. The main advantages of monoliths, apart from short analysis time, are long lifetime and immense robustness, in most cases far exceeding those of particulate columns. This new type of monoliths have at higher efficiency, better peak symmetry and longer lifetime compared with particulate columns.
Sawsan Amer
Cairo University, Egypt
Title: Quality by design approach for development of stability indicating method for determination of cefditoren pivoxil
Biography:
Professor Sawsan Amer completed her PhD in Department of Analytical Chemistry from Cairo University 1985. Her research interests focus on controlled synthesis, separation, assembly and property regulation of functional inorganic nanomaterials and carbon materials. He has published more than 7 publications as the first author or corresponding author in journals including Small molecules from the decomposition of interstellar carbons
Abstract:
A hybrid development strategy of Quality by Design (QbD) and One Factor at Time (OFAT) approaches were used to develop a stability indicating HPLC method for quantitative determination of cefditoren pivoxil (CTP) in bulk powder and pharmaceutical formulations. A forced degradation studies were performed under acid, alkaline, thermal and photolytic stress conditions. Chromatographic separation was achieved in less than 10 min. using a RP C-18 column, mobile phase [methanol: acetate buffer pH 4.5 (55:45, v/v)], flow rate 1.5 mLmin-1 and UV detection at 225 nm. Optimization of column, pH, and wavelength were implemented according to OFAT approach, while elution temperature and methanol content in the mobile phase were implemented considering QbD approach. The method was validated to meet official requirements including specificity, linearity, precision, accuracy and robustness. The drug response was linear (r=0.9999) in range of 89-672 μgmL-1, the limit of detection (LOD) and limit of quantitation (LOQ) were 5.31 μgmL-1 and 16.1 μgmL-1, respectively. The intra and inter-day precisions were 0.11% and 0.44% respectively. The proposed method was successfully applied for the determination of CTP in bulk and tablets with acceptable accuracy and precisions. The results demonstrated that the method would have a great value when applied in quality control and stability studies for CTP.