Day 2 :
- Track 4: High Performance Liquid Chromatography
Track 5: Novel Techniques
Track 8: Product Related Processes
Session Introduction
Gerhard Kratz
Global Sales Director abcr GmbH Im Schlehert 10 76187 Karlsruhe Germany
Title: HPLC – High Performance Liquid Chromatography
Biography:
Gerhard Kratz has completed his studies at University of Applied Sciences in Berlin. His dissertation was about Planning, calculation and construction of a Fluidized bed reactor. Deepening during his studies was Water economy, chemical technology, plastic technology, varnish and paints, oil processing. First positions in industry were in pharmaceutical industry doing HPLC method development on various HPLC brands. Specialized in HPLC column selection and teaching customers in troubleshooting all over the world he is supporting several internet forums for HPLC questions.
Abstract:
Liquid chromatography was defined in the early 1900s by the work of the Russian botanist, Mikhail S. Tswett. His pioneering studies focused on separating compounds [leaf pigments], extracted from plants using a solvent, and in a column packed with particles. Tswett coined the name chromatography [from the Greek words chroma, meaning color, and graph, meaning writing—literally, color writing] to describe his colorful experiment. [Curiously, the Russian name Tswett means color.] Today, liquid chromatography, in its various forms, has become one of the most powerful tools in analytical chemistry. HPLC is the most used analytical testing method for pharmaceuticals and is also used in production to produce ultra-pure pharmaceuticals. For example Insulin, the last purification step is done with preparative HPLC (industrial scale chromatography), at most manufacturing processes! Stationary phases are different due to the different surface chemistry used to do the bonding. High pure Silanes are used to give each packing material its unique performance.
Eduard Rogatsky
Albert Einstein College of Medicine of Yeshiva University, USA
Title: 10 years of evolution in UHPLC-MS chromatography. Current trends.
Biography:
Eduard Rogatsky completed his M.Sc in physical chemistry from Belarus State University, PhD in bioanalytical chemistry from Bar-Ilan University (Israel) in 1999, and postdoctoral studies at Albert Einstein College of Medicine, NY. He joined the faculty there in 2001, and is currently a Senior Associate Scientist and Director of Mass Spectrometry in the Biomarker Analytical Resource Core Laboratory, Einstein-Montefiore Institute for Clinical and Translational Research, Bronx, NY, USA
Abstract:
Ultra High Performance Liquid Chromatography (UHPLC) was introduced in 2004 by Waters. This novel type of liquid chromatography system was designed to utilize resolution power of sub 2 µm particle size columns for fast high resolution separations. Different vendors also have developed novel chromatographic systems. Currently, UHPLC has become a typical chromatographic system, and within the last 10 years both UHPLC practitioners and instrument developers acquire new experience in that field. The author will share his thoughts and findings of utilization UHPLC technology with mass spectrometry detection in clinical settings.
Biography:
Eiji Yashima received his BS, MS, and PhD (1988) from Osaka University. In 1986, he joined Kagoshima University. After a Postdoc with David Tirrell at UMass (1988-1989), he moved to Nagoya University in 1991 and was promoted to a full Professor in 1998. He has published over 300 papers including 23 reviews and has been serving as an Editorial Board or Editorial Advisory Board Member of 10 international journals. His current research interests are in the design and synthesis of helical molecules, supramolecules, and polymers with novel structures and functions
Abstract:
The helix is ubiquitous in nature and one of the prevalent structural motifs for biological polymers, playing key roles in their sophisticated functions, such as chiral recognition, enantio-selective catalysis, and replication. Here, a quite unique helical polyacetylene is shown, whose main-chain helicity and axial chirality of the pendants are induced in the solid state accompanied by significant amplification of the chirality in a sequential or synchronized fashion upon interaction with a nonracemic alcohol. The induced macromolecular helicity and axial chirality are automatically memorized and further switched in the solid state. This unprecedented feature enables in this polymer the switchable chiral stationary phase (CSP) for separating enantiomers by high-performance liquid chromatography (HPLC) whose elution order or enantio-selectivity can be reversibly switched in the column for the first time. Separation of enantiomers by HPLC is now an essential technique for the research and development of chiral drugs in the pharmaceutical industry and a number of CSPs have been commercialized. However, none of them can switch the elution order of enantiomers, which is one of the most important issues to resolve.
Biography:
Imre Sallay was born and raised in Hungary, Budapest. He spent almost four years as researcher/teaching assistant at a local university in the Biochemistry Department. Fate assigned his first task to be HPLC separation of enzyme digested insulin fragments. Moved to Japan with a research scholarship for one-and-a-half years, but got entangled in a PhD course leading to a post-doc binding him irreversibly to the country. The first job at a laboratory supplier company landed him to the chromatography world. For the last 10 years he is travelling around the Globe tirelessly consulting API producer companies with especial attention to insulin manufacturing. He is passionate about the beauty of the science at work in silica based reversed phase chromatography. When not on the road he happily lives in Osaka with his ancient guinea pig.”
Abstract:
As peptide-based APIs are getting more complicated the bar is raised constantly in the downstream purification part. The tool-box available for scientists is bursting, new stationary phases are developed in a steady progress, but most unfortunately often there is no time to do a decent screening for choosing the optimal silica based reversed phases stationary phase for high pressure liquid chromatography. Though the importance is high, usually the last, so called polishing step has strong impact on the process economy. The presentation is moving through the important physicochemical parameters of the silica particles and pointing out the role they play in the process. Special attention is paid for the pore and particle size and how they determine the success of the purification. A sadly neglected point, the great importance of the ligand density or bonding density of the reversed phased silica is explained in details. The presentation is focused on large scale/ process scale purification, but the principles just as much apply to analytical HPLC work. Better integration connecting purification jobs from mg scale up to large industrial scale production is easy to achieve if the scientists manning those different stations have common understanding and common knowledge on the science behind the purification jobs.
Prof Andrew Shalliker
University of Western Sydney, Australia.
Title: New Column Technology Enabling Ultra-High Speed HPLC-MS
Biography:
Professor Shalliker completed his PhD in 1992 from Deakin University, Waurn Ponds, Australia. He completed postdoctoral studies at Queensland University of Technology, Brisbane (Australia) and the University of Tennessee, Knoxville, the latter under the mentorship of Distinguished Professor Georges Guiochon. He is currently a Professor in Analytical Chemistry at the University of Western Sydney and a Deputy Director of the Australian Centre for Research on Separation Science (ACROSS). He has approximately 130 publications.
Abstract:
The suite of chromatography columns referred to as Active Flow Technology (AFT) form the basis of ultra-high speed HPLC-MS analyses. This technology currently comprises three column designs; Parallel Segmented Flow (PSF); the Curtain Flow (CF) and the Reaction Flow (RF) columns. The key benefits of AFT arise from a new design of the outlet end fitting of the column, which enables the separation of the radial central flow from the wall or peripheral flow. This flow ratio can be adjusted (segmentation ratio), establishing virtual columns of almost limitless internal diameter. The benefits of this design effectively revolve around the establishment of wall-less columns, since they function far more efficiently than conventional HPLC columns, especially at high flow rates. As the radial central flow is separated from the peripheral or wall flow, the amount of mobile phase processed by a detection source is reduced. In fact, the amount of flow entering a detector is proportional to the segmentation ratio. Since these columns yield higher efficiency and a reduction in flow to the detector, separations can be undertaken at flow rates that would otherwise be difficult for an MS to process. We have used these columns at flow rates typically around 5 to 6 mL/min with MS detection, but since the outlet fitting segments the flow, just 1 mL/min has been required to be processed by the detector. This presentation details the design of these columns, their operation and their benefits in HPLC separations that incorporate MS detection.
Ali Aboel Dahab
Kings College London, UK
Title: Magnetohydrodynamic Electrophoresis: A novel fully validated method in bio/chemical analysis
Biography:
Dr Ali Aboel Dahab is a researcher and lecturer at the pharmaceutical research division, King’s College London. He is a member of several professional bodies such as Royal society of chemistry and Royal pharmaceutical society of Great Britain. He has published many papers in highly reputable journals and has been serving as an editorial board member of some reputed journals. He has worked as a consultant in the area of biopharmaceutical and toxicological analysis at deltaDot Ltd. He has a special interest in pharmacology, toxicological and chiral analysis, biological spectroscopy and the development of analytical techniques.
Abstract:
There is a continuing need for fully validated and cost effective separation methods to meet the increasingly exacting demands of medicine, pharmaceutical science, industry and the environmental field, covering areas such as proteomics, biotechnology and related sciences. This work sets out to address this issue by radically improving the performance of electrophoretic techniques particularly capillary electrophoresis (CE). Applications are expected in all areas of bio/chemical analysis. The full potential of CE is not being realised routinely because of the relatively poor reproducibility, the adverse effect of EOF (electroosmotic flow) and the non-optimal resolution obtained with molecules like proteins/peptides that can be adsorbed onto the capillary wall. There is a need for greater efficiency and stability in detecting all the components in complicated analytical profiles and better efficiency in achieving the fine separations normally required in chiral analyses. The work described here is based upon the application of magneto-hydrodynamic principles on CE (MHD-CE) with the mathematical principles derived from in-depth considerations of magneto-hydrodynamics' theory (patent pending). Preliminary experiments have proven the concepts and the following benefits have been observed: (1) Shorter migration times with improved repeatability and reproducibility; (2) Improved peak profiles, (3) Reduction in diffusion (D) and electric double layer (The double layer thickness or Debye ionic radius (ï¤); Reduced EOF and improved electrophoretic mobility, (4) Improved current density, (5) Improved efficiency and resolution. (6) The mathematical basis for the phenomenon is appropriate. It is hoped through the work on MHD-CE that an all-encompassing theoretical frame work will be established and MHDE gives the technique the push it has long needed.
Jaya Vejayan
University Malaysia Pahang, Malaysia
Title: Two Dimensional Electrophoresis (2DE) Guided Purification Novel Technique in the Isolation of Bioactive Proteins from Complex Mixtures
Biography:
Jaya Vejayan has completed his PhD from University Malaya, a premier university in Malaysia. In his MSc he was involved in isolating bioactive compounds from the medicinal plant, Ipomea pes caprae, known to be an antitoxin to jellyfish toxins. While in his PhD he used proteomics to study proteins in various snake venoms in Malaysia. Accordingly, he merged both knowledge together to derive the 2DE guided purification technique.
Abstract:
There are still many laboratories in the East which use the conventional two dimensional electrophoresis. Progressive developments in liquid chromatography and mass spectrometry in recent years led to the obvious demise of 2DE in the West. Nevertheless, due to its robustness, low cost in acquiring and maintenance, 2DE used widely in countries like Malaysia. Efforts required to be done to provide importance to this system. One such effort is been highlighted in this paper. In the natural product research, to isolate bioactive compounds from medicinal plants, an assay will be used to detect presence of the compound within fractions after each sequential chromatography techniques such as silica gel chromatography or HPLC. This technique that will eventually lead to pure compound is known as Bioassay Guided Isolation. Adopting the principle of this technique, 2DE guided purification technique has been derived in the field of proteomics. The technique is simple whereby in place of bioassay will be a 2DE mapped database of a complex mixture such as snake venom. Next, by performing the common separating techniques such as size exclusion or ion exchange or others, fractions will be screened for presence of protein of interest. The fractions showing obvious presence of protein or its subunit stained spots in 2DE will be accepted for further fractionation. The steps will be repeated till homogeneity is achieved. This technique will be illustrated accordingly by the isolation of rhodocetin. In this example, rhodocetin a potential therapeutic protein contained in Calloselasma rhodostoma snake venom will be purified successfully using 2DE guided purification technique.
Ekwere Mercy R
University of Calabar, Nigeria
Title: Thin layer chromatographic analysis of capsaicin and moisture contents from three varieties of capsicum peppers
Biography:
Ekwere Mercy R is a Senior Lecturer and a PhD student. He has a teaching and research experience in Biochemistry for 20 years.
Abstract:
Levels of pungency of capsicum species depends on the concentration of alkaloid compound, capsaicinoids, primarily of capsaicin found in the fruit and only in the plant genus, capsicum. In detecting capsaicin, taster fatigue occurs and tasters are not able to distinguish between the different capsaicinods, therefore the organoleptic test has been replaced with instrumental methods. The work sought to employ a cheap, affordable and readily available method. Crude capsaicin extracted previously from three varieties of capsicum genus [Bird eye pepper, X1- (Capsicum frutescens), chilli pepper -X2 and sweet pepper -X3 (Capsicum annum)] by a modified method of Kosuge et al. (1958) were separated and identified by means of thin layer partition chromatograph impregnated with silica gel G plates (SiO2) and developed in a closed chamber with 95% methanol. The pungent fractions for bird-eye and chilli peppers showed dominant spots at Rf 0.59±0.21, 0.95±0.03 and 0.67±0.01, 0.92±0.10 respectively. While bird-eye pepper had a third spot at Rf 0.93±0.00, a third spot for chilli pepper was not detected. However, sweet pepper had a spot at Rf 0.82±0.02, similar to the third spot as that of bird – eye. The separated fractions were identified by comparing their Rf values with those of authentic capsaicin standards and literature data. TLC method appears to be adequate for routine analysis and could suffice as an essential preliminary purification of capsaicin where scare equipment and most reagents are unavailable.
Asokan C
Sokoto State University, Nigeria
Title: Enhanced cleavage of amyloid ï¢ peptides in the casein injected inflammatory brain of mice
Biography:
Asokan C has completed his PhD from University of Madras and Postdoctoral studies from Columbia University, NY, USA. He is an Associate Professor at the Department of Biochemistry, Sokoto State University, Sokoto, Nigeria. He has published more than 36 papers in reputed journals and has been serving as an Editorial Board Member of repute.
Abstract:
The excessive accumulation of amyloid β-Peptides (Aβs) in the brain is the causative factor in all genetic as well as sporadic cases of Alzheimer’s disease (AD). Two enzymes namely beta-secretase and gamma-secretase are involved in the defective cleavage of Amyloid Precursor Protein (APP) and the alpha-secretase is involved in its normal processing. The core protein of the amyloid senile plaques within the brains of afflicted individuals contains peptide of 39-43 residues, but mostly terminating with residues 40 and 42. The longer Aβ42 is more abundant in the amyloid of the neuritic plaques while the shorter peptides (Aβ40) found more in the vascular deposits. Factors that lead to the over expression of Aβs are yet to be identified. In the present study, we have shown that the prolonged subcutaneous injection of casein, which is known to develop organomegaly of the liver, spleen and kidneys in mice (the Congo red stain of the liver confirmed the presence of a fibrillary protein deposits with amyloid characteristics in chronic inflammation and associated systemic amyloidosis) triggers about 20 times more Abeta accumulation (in sixty six days period) in the mice brain than that of the control mice which was confirmed as well as quantified by RP-HPLC. From the Mass Spectroscopic analysis, we have shown the occurrence of a new type of proteolytic zeta cleavage fragment, a 1-54 residue Abeta in the mice brain along with the Abeta peptides.
Gerard Rosse
Dart NeuroScience, San Diego, CA,
Title: Advances and Applications of Supercritical Fluid Chromatohgraphy in Drug Discovery
Biography:
Rosse is currently Associate Director, Structure Guided Chemistry, at Dart Neuroscience and serves as Adjunct Associate Professor at Drexel University. Prior he functioned in leadership and scientific positions in medicinal chemistry with Cephalon, Sanofi and F. Hoffman-La Roche. During his industrial tenure, he led multidisciplinary teams and invented pre-clinical candidates for a wide range of therapeutic indications including CNS, Inflammation, Metabolism, Oncology, and Antibacterial agents. Dr. Rosse’s career is also characterized by the implementation of innovative chemical technology and analytical chemistry platforms that accelerated the drug discovery process and reduced costs. He received the Ph.D. degree in chemistry from the University of Basel in Switzerland and postdoctoral training at Stanford University, California.
Abstract:
Innovative technologies to make better compounds and make them faster have been implemented. The presentation will focus on the design of a highly automated facility with a multistep process for the rapid analysis and purification of small molecules using Supercritical Fluid Chromatography-Mass Spectrometry (SFC-MS). The recent introduction of a new generation of SFC-MS instruments is revolutionizing the field of chromatography. The instrumentation and the robotic infrastructure to support the production of ~10,000 compounds/month will be discussed. Finally the benefits of an enhanced SFC-MS technology platform to increase productivity and decrease costs will be discussed.
Gaetane LESPES
University of Pau (UPPA), France
Title: Characterization of nanoparticles: how to meet the multiple challenges?
Biography:
Gaetane LESPES has completed his PhD at the age of 26 years from Pau University and a postdoctoral stay from Plymouth University. She founded and chairs the G-4F, Scientific Group of Field-Flow Fractionation that promotes the development of innovative approaches for nano- and micro-characterization in material sciences, medicine, food, and environment. She has published more than 75 papers in reputed journals and has been serving as an editorial board member of American Journal of Analytical Chemistry.
Abstract:
The development of nanotechnologies inevitably induce the need for analytical methods capable of characterizing nanomaterials such as nanoparticles and nanotubes. The challenge remains important due to the diversity in size, shape, chemical composition of natural and engineered nano-objects combined with the complexity of the surrounding media. Some years ago, Field-Flow Fractionation (FFF) coupled with UV, Multi-Angle Light Scattering (MALS) and atomic mass spectrometry (ICPMS) has been proposed as a powerful analytical strategy for such challenge. In this presentation FFF will be presented from its principle to its analytical performances. Different examples of applications in material sciences and environmental media will be presented in order to illustrate the capabilities of FFF-multidetection.
Biography:
Zahir Akhunzada is a PPD Consultant in the Analytical & Bioanalytical Development department at Bristol-Myers Squibb in New Brunswick, New Jersey where he is responsible for the analysis and characterization of proteins by MFI. Before joining PPD, he worked for Schering-Plough/Merck. He was Assistant Prof. at King Saud University Riyadh Saudi Arabia and a Guest Investigator at the VA Hospital in Newark, NJ. He has a broad range of expertise in R&D, spectroscopy, chromatography, wet analytical techniques and biologics. He acquired research experience in the Netherland, Pakistan and earned his PhD in Chemistry in 1992. He did his Post doctorate in Germany, has several research publications and co-authored, a textbook with Prof. Atta-ur-Rahman on “Streoselective Synthesis in Organic Chemistry”, published (1993) by Springer Verlag, New York.
Abstract:
The presence of sub-visible particles (SVPs) is a major challenge in the development of therapeutic protein formulations. Distinction between proteinaceous and non-proteinaceous SVPs is vital in monitoring the formulation stability. The current compendial method based on light obscuration (LO) has limitations in analyzing translucent particles, requires large analysis volume and therefore demands urgent need for an unambiguous method to characterize SVPs. A number of attempts have been made to characterize SVPs, albeit with limited success. This presentation reveals a method that successfully characterizes and distinguishes, both potentially proteinaceous and non-proteinaceous SVPs in protein formulations by using Microflow Imaging (MFI) in conjunction with the MVAS (MFI View Analysis Suite) software.
Fernanda Costa
Universidade Federal do Rio de Janeiro, Brazil
Title: Solvent System Selectivities in Countercurrent Chromatography Using Salicornia gaudichaudiana Metabolites as Practical Example with Off-line Electrospray Mass-Spectrometry Injection Profiling
Biography:
Fernanda das Neves Costa has completed her PhD at the Universidade Federal do Rio de Janeiro with an internship at Technische Universität Braunschweig (Germany) and Brunel University (England). Now she is an assistant professor at the same Univeristy in Rio de Janeiro, Brazil. She has published more 12 papers until the present moment.
Abstract:
Salicornia gaudichaudiana (Chenopodiaceae) is a halophyte plant that grows in high-level salt soil. Plant material is used as ‘green salt’ in food preparations for people with high blood pressure and kidney / heart diseases. Countercurrent chromatography (CCC) is a form of liquid-liquid partition chromatography in which the stationary liquid phase is retained in the apparatus without the use of a solid support. A large variety of solvent systems (SoSy) have been proposed and employed in CCC which, despite being an efficient technique, will not separate compounds of a complex mixture without the appropriated system. This study describes the influence of SoSy selectivity on the separation of S. gaudichaudiana metabolites. HEMW at 0.5:6:0.5:6 and HBuWat 1:1:2, medium polarity SoSy, gave good distribution of compounds between the two phases. Two CCC runs were performed at identical experimental conditions using the two SoSy. Odd fractions were analyzed by decoupled ESI-MS/MS for metabolite monitoring. Selected ion traces in the two reconstituted CCC-ESI-MS/MS allowed the visualization of the SoSy selectivity for major compounds. In general, HEMW at was more selective and distributed all compounds along the CCC separation with isolation of flavonol glycosides, although co-elution of dicaffeoylquinic acids occurred. HBuWat with less general compound selectivity fractionated isomeric caffeoyl-quinic acids. The SoSy selectivity in CCC is very important when having a target compound in a complex mixture. The strategic use of different SoSy in a specific sequence can influence the results. From the results of our study we could plan to isolate the flavonoids with HEMW at SoSy and then re-fractionate the sample with HBuWat for the isolation of caffeoyl-quinic acid isomers.
- Track 12: Large Scale Preparative Chromatography for the manufacturing of APIs
Session Introduction
Olivier Dapremont
AMPAC Fine Chemicals LLC, USA
Title: Implementation of a continuous chromatographic process for the manufacturing of an API
Biography:
Olivier Dapremont is a renowned expert in the implementation of continuous chromatography (SMB) for the purification of APIs. He started in 1992, developing the SMB technology for Prochrom in France, and then joined Chiral Technologies to manage the kilo separation laboratory using SMB. He joined AMPAC Fine Chemicals (then Aerojet) in California, in 2001 to be in charge of the development of continuous processes. He has developed more than 50 SMB separations at all scale. He is a member of the Prep Symposium Organizing Committee as well as a member of the SPICA Scientific Committee.
Abstract:
The advantages of continuous processing for commercial scale manufacturing are well known and accepted in many industries; reduction in the manufacturing footprint, increase in process safety, increased automation, reduction in manpower requirements, better economics are some of the known advantages. However, implementing a continuous process in an industry that is traditionally batch is often challenging. The regulatory aspects are certainly the most difficult to overcome as a shift in the approach to validation, process monitoring and even “batch” documentation is required to adapt to the specificity of the continuous nature of the process. The author will discuss the various aspects of the successful implementation of a continuous chromatographic process for the manufacturing of an API at commercial scale through actual examples.
Joseph Barendt
Chiral Technologies, Inc., USA
Title: Modern techniques for enantiomeric separation: From grams to tons
Biography:
Joseph Barendt earned his PhD in Chemistry at the University of Colorado, Boulder, followed by Postdoctoral work at UCLA. His career has been devoted to applying interdisciplinary technologies to improve organic synthetic routes for pharmaceutical products. Among his earlier accomplishments are the first commercial launches of enantioselective reducing agents and catalysts for the industry. In his current role as Chief Operating Officer of Chiral Technologies, he is responsible for all aspects of chiral chromatography, including analytical, kilo-lab separations, and metric ton quantities.
Abstract:
With ever-increasing complexity in pharmaceutical products, and the need for enantiomerically pure materials, today’s chemists are moving toward a “toolbox” approach in their research. The best toolboxes are those with the broadest range of tools to ensure that the every job can be completed. This presentation will use several case studies to explore various tools at the disposal of chemists seeking single enantiomers. Further, we will consider the unique constraints at every stage of the pharmaceutical process to better understand the proper selection of these tools from discovery through clinical trials.
Rob Driscoll
Robatel Inc., Pittsfield, MA
Title: Fast Centrifugal Partitioning Chromatograph for Extract Purification
Biography:
Rob Driscoll is a Senior Application Engineer at Robatel Inc. and has worked in the pharmaceutical and fine chemicals industries for 19 years. He is responsible for the start-up and optimization of centrifugal equipment and is responsible for the Kromaton centrifugal chromatography activities. He has a BS in Chemical Engineering from Michigan State University.
Abstract:
Centrifugal Chromatography has been developed to select and recover desired compounds at analytical, preparative, pilot, and industrial scales. Centrifugal portioning chromatography, a biphasic liquid process technology, is used for the fractionation and purification of biologic matrices, synthetic solutions, and natural products. According to the polarity and solubility of the desired molecule, FCPC permits the selection and recovery of desired compounds while achieving up to 99% purity levels. With no costly solid packing material or columns to replace, purification by FCPC reduces batch to batch costs and eliminates the risk of contamination between products. Assuring 100% recovery of samples, liquid-liquid centrifugal partition chromatography is extremely flexible allowing for both ascending and descending modes of operation with no loss of compounds due to adsorption, and no denaturing of sensitive molecules
Biography:
Marc Jacob joined Phenomenex as Global Product Manager for Chiral and Preparative Chromatography in 2011. Previously, he was Director of Process Development at Bachem (Torrance, USA) where he led efforts to develop and produce peptide Active Pharmaceutical Ingredient. Prior Bachem, he works for contract manufacturing organizations in the research, development and manufacturing of small molecules such as chiral amino acids and peptide building blocks. Jacob earned his Doctorate in Synthetic Organic Chemistry at the University of Montpellier
Abstract:
Over the last few decades, peptide synthesis technology has developed to allow for large scale manufacturing of peptides. The interest in using peptides as therapeutic agents has been a significant driver of this technology. Separation and isolation technology of the desired peptide product have also been developing along with the synthesis. The purification process typically includes chromatography separation for purifying the peptide active ingredient from crude synthetic peptide mixtures. A single step chromatographic process is most desired but a multiple step process is often necessary. When a multistep process is used, the first step is designed to isolate the desired material by removing most of the undesired components. The isolated material is then purified further by one or more different chromatographic steps to “polish” the material to the desired purity level. This presentation will cover the current state for large scale peptide manufacturing with focus on HPLC purification. The chemical and physical property of the purification packing material will be also discussed. Few example of peptide pharmaceutical ingredient with more than 20 amino acids will be presented.
Ammar Altemimi
Southern Illinois University at Carbondale, USA
Title: In-vitro antibacterial activity of optimized spinach extraction for food safety and preservation
Biography:
Ammar Altemimi worked as Lecturer (2005 - 2011) in Department of Food Science and Biotechnology, University of Basrah, Iraq. He taught biochemistry and biotechnology for undergraduate, food chemistry, dairy products. He is into: Developing academic programs, monitoring students’ educational progress, train and motivate other non-teaching staff, managing career counseling and other student service. He has published more than 5 papers in reputed journals such as Ultrsonic Sonochemistry Journal and Molecules Journal and has been serving as an Editorial Board Member and Reviewer of repute journals.
Abstract:
The aim of this study was to explore the potential antimicrobial activities of the optimized spinach extracts on Gram-negative and Gram-positive food-borne bacteria and evaluate the antimicrobial effect at the molecular level. The results indicate that the optimized spinach extract exhibited the antimicrobial activity against tested microorganisms. Moreover, the results obtained after the treatments of bacterial strains with elevated concentrations of the optimized spinach extracts revealed that the extract had potent lethal activities as the growth turbidity decreased as the concentration or time of exposure increased. In addition, the observation by the scanning electron microscope showed that cells of the bacterial strains were damaged after the treatment with the optimized spinach extract. The antimicrobial effect was explored at the molecular level, using random amplification of polymorphic DNA (RAPD) analysis of the genomic DNA extract from the control (untreated) and the optimized spinach extract -treated bacterial strains. The results demonstrate that the polymorphic bands were decreased for most treated microbes compared with untreated strains. All these results strongly point out the mutagenicity, lethal and antimicrobial effect of the optimized spinach extracts. The results indicate the possibility of using the leaves extract of spinach as a source of antibacterial compounds for treatment of infections caused by bacterial pathogens.
Zhuomin Zhang
Sun Yat-sen University, China
Title: Development of novel enrichment media of trace volatiles for real sample analysis
Biography:
Dr. Zhuomin Zhang has completed his PhD in 2007 from Sun Yat-sen University majoring in analytical chemistry. Now, he is an associate professor in Sun Yat-sen University. His research interests are sample preparation and chromatographic analysis. The main research field covers the development of novel enrichment media and device for gas sampling project and chromatographic analytical method for bio-odoromics. As a young scholar, he has published more than 40 papers in reputed academic journals.
Abstract:
Gas sampling techniques, especially for the enrichment and separation of trace gases from complicated matrix still remain one of the weak spots in analytical chemistry nowadays due to gas strong volatility, compared with liquid and solid sample. Efficient gas sampling techniques should possess the highest extraction capacity and selectivity and can be conveniently coupled with sequent analytical instruments. The core of developing efficient gas sampling techniques lies in the development of new enrichment media with higher extraction capacity and selectivity. In our previous reports, a series of novel enrichment media, including one-dimensional nano-arrayed, conjugated doping and Metal Organic Frame (MOF)-based coatings were developed, which mainly focused on the improvement of sampling capacity and selectivity for trace target gases from real samples with complicated matrices. The fabrication formats of these novel enrichment media, mainly involved sorbent tube, headspace stir bar and Solid-Phase Micro Extraction (SPME) coatings coupling with the consequent analytical instruments. These novels trace gas enrichment media have been successfully applied for the enrichment and analysis of trace target volatiles from food, environmental and biological samples.