Dr Imre
Osaka, Japan
Title: Advances in HPLC Purification of Peptides – Future Benefits of In-house Integration
Biography
Biography: Dr Imre
Abstract
As peptide-based APIs are getting more complicated the bar is raised constantly in the downstream purification part. The tool-box available for scientists is bursting, new stationary phases are developed in a steady progress, but most unfortunately often there is no time to do a decent screening for choosing the optimal silica based reversed phases stationary phase for high pressure liquid chromatography. Though the importance is high, usually the last, so called polishing step has strong impact on the process economy. The presentation is moving through the important physicochemical parameters of the silica particles and pointing out the role they play in the process. Special attention is paid for the pore and particle size and how they determine the success of the purification. A sadly neglected point, the great importance of the ligand density or bonding density of the reversed phased silica is explained in details. The presentation is focused on large scale/ process scale purification, but the principles just as much apply to analytical HPLC work. Better integration connecting purification jobs from mg scale up to large industrial scale production is easy to achieve if the scientists manning those different stations have common understanding and common knowledge on the science behind the purification jobs.