Alessandra Serain is a currently a Pharmacist from the State University of Campinas and a PhD candidate with FAPESP scolarship, in the Biosciences and Technology Program of Bioactive Products of the DBV / IB - UNICAMP with the research project that seeks to perform a phytochemical study of two species of Sinningia for application in Photodynamic Therapy (PDT) in Culture of human cells, and biotechnological study of plant cell culture. Has experience of Scientific Initiation at BTPB-UNICAMP, 18 months with FAPESP scholarship, in the field of phytochemistry, PDT, essential oils and microbiological activity
The Sinningia magnifica belong to Gesneriaceae family what comprises around 150 genera, distributed around the world. Phytochemical studies with Sinningia reported the identification of phenolic glycosides, anthocyanins and anthraquinones with biological properties (Scharf et al., 2016). The Photodynamic chemotherapy (PDT) has been used for many purposes, such as antitumor, performing as a non-invasive therapy with minimal side effects using photosensitizers that, under illumination at a certain wavelength and in the presence of oxygen, produce reactive oxygen species, cytotoxic to the target cells (Brown et al., 2004; Andreazza et al., 2015). In this initial work, was made a cromatographic fractionation using silica as stationary phase by flash column, open column and thin layer cromatography of an ethanolic extract of S. magnifica prepared by maceration of its tubers. This fractions and isolated compounds of our research group was used to an investigation of the potential for employment as natural photosensitizer against human prostate cell line (PC-3) and a control cell line (3T3). Also, was determined the absorption profile of isolated compounds in the visible region, and the 1,3-DPBF decay, as an indirect method of singlet oxygen generation, of the best compounds. The antiproliferative activity was assessed by the reduction of a tetrazolium salt (MTT), carried out in triplicate, using an irradiated plate and a non-irradiated plate in sub-inhibitory concentration. The irradiated plate reduced the cell viability comparing with non-irradiated plate. Despite the positive results, further investigations are necessary to confirm the potential of this technique and the bioactive compounds as PDT agent.
Ada Saccà is a chemist. She completed his PhD in Materials for the environment and energy from TorVergata University (Rome, Italy) in 2006, 2nd level Master’s degree in Hydrogen production systems and means of transport with fuel cells from Messina University (Italy) in 2008. She is actually researcher and has a permanent position at National Research Council of Italy, Institute for Advanced Energy Technologies Nicola Giordano (Messina, Italy). Her activity regards Components for PEFC, Fillers for PEM and Electrodes, Membranes for Electrochemical Devices and, recently, for Separation Processes. She published 32 papers on international journals, 73 on abstracts books, 53 reports.
In the sustainable global development context, the increasing demand of water for human requirements favored the research on alternative wastewaters purification processes. In the last 50 years, new technologies for substances separation, so-called membrane processes (Microfiltration, Nanofiltration, Ultrafiltration, Osmosis), have been extensively studied. They use different membranes and operative conditions promoting the separation and removing contaminant particles in a wide molecular cut-off range. Ultrafiltration process, used for water treatment, works in low-pressure conditions. One of the most used polymers is Polysulphone (PSF) due to its high thermal, chemical and hydrolytic stability. In the present work, a preliminary investigation on PSF membranes with hydrophilic and antifouling properties is presented. The phase inversion method by non-solvent coagulation-bath-technique was used to obtain asymmetric membranes with different characteristics of porosity, hydrophilic properties, macro-voids size and shape. The following parameters were considered: not-solvent kind, de-mixing time, polymer concentration, surfactant insertion/concentration. The membranes were characterized in terms of XRD, TGA-DSC, dimensional analysis, water retention, qualitative contact-angle, DMA. In particular, cross-section SEM was used to evaluate the relationship between membrane formation and obtained morphology, pursuing the finger-like macro-voids morphology, suitable for final application. Promising results in terms of compromise morphology/hydrophilic properties/stiffness were obtained and some of these samples permitted having a good approximation for finger-like morphology, aspect to further investigate. Permeability tests, contact angle and zeta potential measurements are actually in progress in order to individuate the most suitable procedure and the most performing sample for application in the industrial wastewaters purification and recovery of contaminating species.