Separation Techniques

Biography

Biography: Sena Caglar

Abstract

In pharmaceutical, bioanalytical and biomedical analysis of drugs and metabolites in biological fluids is essential for bioequivalence/bioavailability, therapeutic drug monitoring and drug abuse studies. An optimal and effective sample preparation method plays the most important role since the depletion of the matrix in biological fluids is the biggest issue for a trouble-free analysis. It is impossible to inject the biofluid directly to the chromatographic system with traditional methods but this challenge was overcome by the on-line methods. Combination of solid phase extraction (SPE) with high performance liquid chromatography allows direct analysis of small molecules (i.e., drugs) in biofluids. The method, in summary, depends on connection of the SPE column, coated with various packing materials, directly to the analytical column (where the analytes are separated) through a switchingvalve and injection of the sample to the system. Following the injection, i) the matrix components are depleted and ii) the analytes are separated in the column. Thus, sample preparation step from the biological fluid is completely eliminated and the sample can be directly injected to the system resulting with high reproducibility in the analyses.