Gerhard Kratz
Global Sales Director abcr GmbH Im Schlehert 10 76187 Karlsruhe Germany
Title: HPLC – High Performance Liquid Chromatography
Biography
Biography: Gerhard Kratz
Abstract
Liquid chromatography was defined in the early 1900s by the work of the Russian botanist, Mikhail S. Tswett. His pioneering studies focused on separating compounds [leaf pigments], extracted from plants using a solvent, and in a column packed with particles. Tswett coined the name chromatography [from the Greek words chroma, meaning color, and graph, meaning writing—literally, color writing] to describe his colorful experiment. [Curiously, the Russian name Tswett means color.] Today, liquid chromatography, in its various forms, has become one of the most powerful tools in analytical chemistry. HPLC is the most used analytical testing method for pharmaceuticals and is also used in production to produce ultra-pure pharmaceuticals. For example Insulin, the last purification step is done with preparative HPLC (industrial scale chromatography), at most manufacturing processes! Stationary phases are different due to the different surface chemistry used to do the bonding. High pure Silanes are used to give each packing material its unique performance.