Marc Jacob
Phenomenex, USA
Title: Large scale HPLC purification of peptides: The art of using spherical silica based packing material for producing large biomolecule with high purity
Biography
Biography: Marc Jacob
Abstract
Over the last few decades, peptide synthesis technology has developed to allow for large scale manufacturing of peptides. The interest in using peptides as therapeutic agents has been a significant driver of this technology. Separation and isolation technology of the desired peptide product have also been developing along with the synthesis. The purification process typically includes chromatography separation for purifying the peptide active ingredient from crude synthetic peptide mixtures. A single step chromatographic process is most desired but a multiple step process is often necessary. When a multistep process is used, the first step is designed to isolate the desired material by removing most of the undesired components. The isolated material is then purified further by one or more different chromatographic steps to “polish” the material to the desired purity level. This presentation will cover the current state for large scale peptide manufacturing with focus on HPLC purification. The chemical and physical property of the purification packing material will be also discussed. Few example of peptide pharmaceutical ingredient with more than 20 amino acids will be presented.