Pao-Chi Liao
National Cheng Kung University, Taiwan
Title: Metabolomics approaches for bio-marker discovery for toxicant exposure of di-isononyl phthalates (DINPs) using liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Biography
Biography: Pao-Chi Liao
Abstract
Di-Isononyl Phthalate (DINPs) is widely used as plasticizers and has effects on reproductive system. Three metabolomics approaches were used to explore DINP exposure markers in an LTQ/Orbitrap HRMS dataset obtained from liver enzyme incubation. The data processing techniques included Signal Mining Algorithm with Isotope Tracing (SMAIT), Mass Defect Filtering (MDF), and web-based XCMS. Fourteen metabolites were validated as DINP exposure markers using a rat model. Among the 14 exposure-related DINP metabolite signals, 8 have not been reported in the literature. The metabolomics platform can efficiently and systematically filter probable metabolite signals from a complex LC-HRMS dataset for toxic exposure marker discovery.