Solich Petr
Charles University, Czech Republic
Title: Trends in development of stationary phases in chromatography
Biography
Biography: Solich Petr
Abstract
High performance liquid chromatography is one of the most progressive separation techniques which allows identification and quantification in one step and is frequently used in clinical research for analysis of biological samples in connection with modern sample preparation. Choice of the sample preparation technique is key step which influence sensitivity, robustness, solvents and sample consumptions etc. Connection of modern, fast and robust sample preparation procedures and modern trends in liquid chromatography are useful base for clinical research. A huge expansion of new stationary phases was registered during last couple of years. Several different technologies with different characteristics were introduced into the market, among them Core shell technology using porous shell and solid core particles. These columns can be used in common HPLC instruments as well as in UHPLC systems. This technology promises to increase of resolution and maximizes throughput, and result in solvent saving and easier method transfer. The recently introduced HR monolithic technology is based on a unique sorbent material allowing good quality of separations in a minimal time. The main advantages of monoliths, apart from short analysis time, are long lifetime and immense robustness, in most cases far exceeding those of particulate columns. This new type of monoliths have at higher efficiency, better peak symmetry and longer lifetime compared with particulate columns.