Dina Shokry
University of Huddersfield, UK
Title: Novel techniques for predicting human intestinal absorption
Biography
Biography: Dina Shokry
Abstract
Estimation of human intestinal absorption is very important especially for orally administered pharmaceutical compounds with poor solubility. Therefore, in preformulation studies the extent of drug absorption must be determined for new drug entities (NDE). The use of animals has been the most abundant method used in preformulation studies for determination of pharmacokinetics, especially the rate and extent of intestinal absorption of pharmaceutical compounds. In this work, using the biodetergent-based micellisation with a form of chromatography known as Micellar Liquid Chromatography and the use of the solubilizing capacity of these biodetergents and its determination using UV-spectrophotometry were found to be successful in the prediction of human intestinal absorption. Bile salts were used as a mobile phase in the MLC chromatographic method to provide an environment more closely simulating the human intestinal environment. In this method, intestinal absorption was successfully predicted by the use of a group of model compounds through measurement and calculation of the partition coefficient, Pmw. While in the spectrophotometric method the solubilizing effect of one bile salt was quantified and used in the calculation of the partition coefficients (log Kxm/a) of a number of model drugs which were then used in the construction of a statistical model for the prediction of human intestinal absorption. Another method used for prediction of human intestinal absorption was a permeation method using flow through or Franz cells using drug-sodium deoxycholate based polymer where the permeability constant Kp was obtained for number of drugs and used in statistical modelling of human intestinal absorption.