Feng Zhang
Chinese Academy of Inspection & Quarantine, China
Title: A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and metabolites in serum samples by ultra-high performance supercritical fluid chromatography
Biography
Biography: Feng Zhang
Abstract
Sulfonamides are a large group of synthetic antibiotics which has anti-bacterial properties. They have a good antibacterial effect on both Gram-positive and Gram-negative bacteria. Sulfonamides are commonly prescribed in human and veterinary medicine against many kinds of infections. In order to investigate the pharmacodynamics and pharmacokinetics of different sulfonamides, establishing a detection method of multiple sulfonamides and their metabolites in serum is necessary. An analytical method based on ultra-high performance supercritical fluid chromatography (UHPSFC) with photo-diode array detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Under the optimized gradient elution conditions, it took only 7 min to separate all 15 sulfonamides and the critical pairs of each parent drug and metabolite were completely separated. Variables affecting the UHPSFC were optimized to get a better separation. The performance of the developed method was evaluated. The UHPSFC method allowed the baseline separation and determination of 15 sulfonamides and metabolites with a limit of detection ranging from 0.15 to 0.35 g/mL. Recoveries between 90.1 and 102.2% were obtained with satisfactory precision since relative standard deviations were always below 3%. Hence, the proposed method is simple, accurate, time-saving and green; it is applicable to a variety of sulfonamides detection in serum samples.