Separation Techniques

Recent Advances in Chromatography-HPLC

The HPLC method enables the identification of a large variety of metabolites when biological samples are being analysed. Two human embryo culture media samples were examined using high-pressure liquid chromatography-mass spectrometry (HPLC-MS). They are based on the hypothesis that a variety of microorganisms each have a unique mass spectral signature based on the proteins and peptides found in the cells. It is challenging to identify unknown peaks in GC-MS-based discovery metabolomics, but it is vital to locate novel or unexpected metabolites that may be implicated in allergic disease processes and/or to increase our understanding of how genotypes affect phenotypes. Here, we'll take a closer look at two novel approaches to peak detection and recent advancements in pharmaceutical analytical techniques.