Day 1 :
Keynote Forum
Iakovos Yakoumis
Monolithos Catalysts and Recycling Ltd, Greece
Keynote: Towards hollow fibers automotive catalytic converters: effect of carbon on the NO abatement efficiency of Cu decorated C/Al 2 O 3 porous hollow fibers
Time : 09:15-09:40
Biography:
Iakovos Yakoumis was born in 1974 in Athens. He obtained his MSc degree in Chemical Engineering from the National Technical University of Athens in 1997. He has published 14 research papers in international scientific journals. The citation index, of these papers, is more than 1042 (Google Scholar). He is the Founder and the Managing Director of Monolithos Catalysts and Recycling Ltd. In 2000, he was honored for the Organization of the Greek Small and Medium Enterprises with the Panhellenic Award of the Best Young Businessman of the Year. He was Member of the Board of several local and business associations. He has served as elected Vice-President of the City Council of Naxos and Small Cyclades Municipality (2014).
Abstract:
In this work, biopolymer/ceramic hollow fibers were manufactured at high yield using a modified polyol process in spinneret set-up and allowed to adsorb controllable amounts of Cu2+. The fibers were further converted to catalytic Cu decorated, carbon/ceramic composite hollow fibers (C/Al2O3) by a post-sintering technique. The polyol process modification pertained to the use of alginate as the metal ion binder and metallic nanoparticles stabilizer. The walls of the hollow fibers were porous, exposing a high surface area decorated with Cu nanoparticles. The structural and morphological properties of the obtained catalytic composite hollow fibers have been studied and their DeNOx abatement efficiency has been evaluated via a continuous flow process we propose here, with the gas stream sweeping the shell and lumen side of a bundle of the fibers in the tangential flow mode. The stability, long working-life and easy regeneration of the composite catalytic fibers were studied in relation to the carbonaceous content and the possible deactivation/reactivation mechanisms. It has been concluded that carbon contributed significantly to the improvement of the DeNOx activity, especially in the cases, where reducing gases such as CO were absent from the gas stream. Moreover, the DeNOx efficiency was high and stable for more than 300 hours on stream, a feature which combined with the viability in terms of manufacturability and yield, makes us propose these catalytic fibers and the respective bundle type reactor as the next generation technology for NO abatement.
Keynote Forum
Dusan Berek
Slovak Academy of Sciences, Slovakia
Keynote: Liquid chromatography of block copolymers
Time : 09:40-10:05
Biography:
Dusan Berek, PhD, DSc is employed at Polymer Institute, Slovak Academy of Sciences in Bratislava. He served as Elected Member of the Presidium of the Slovak Academy of Sciences, President of the Slovak Chemical Society, and Chairman of the Czecho-Slovak and Slovak National Committee of Chemistry for IUPAC. He is Corresponding Member of the Central European Academy of Sciences and Member of the Learned Society of the Slovak Academy of Sciences. He is Author or Co-author of two monographs and 250+ scientific papers in extenso published in refereed periodicals, proceedings and chapters of books, as well as 60+ patents (four of them were licensed) and was cited more than 2,000 times. He presented over 110 invited plenary, key and main lectures, as well as over 900 regular lectures and poster contributions on symposia and conferences and during lecturing tours to over 40 countries. He was elected as “Slovak Scientist of the Year 1999” and “Slovak Innovator of the Year 2001”.
Abstract:
Block copolymers represent an important group of materials with extensive applications in science, medicine and technology. In a block copolymer, at least two chemically distinct polymer chains are connected with a chemical bond. Comprehensive molecular characterization of block copolymers represents an analytical challenge. A special problem is determination of amount and molar mass of parent homopolymers, which are present in most block copolymers, and which constitute highly undesired, expensive ballast. Gel permeation chromatography, (size exclusion chromatography) GPC/SEC is commonly employed for characterization of block copolymers. Molar mass of a precursor, the block polymerized as first, is determined by GPC/SEC and the same method is employed for the approximate assessment of total molar mass of block copolymers. Due to low separation selectivity and detector sensitivity GPC/SEC size exclusion chromatography can hardly identify presences and render molar mass information on parent homopolymers. We will discuss principles and applications of the original alternative liquid chromatography methods namely liquid chromatography under limiting conditions of enthalpic interactions, LC-LC and sequential two-dimensional polymer liquid chromatography, S2D-LC. LC-LC methods are well robust and experimentally feasible. Their separation selectivity is very high and sample recovery is reasonable. LC-LC can in one single step easily and efficiently discriminate both parent homopolymers from diblock copolymers. The separated sample constituents can be one-by-one forwarded into an on-line GPC/SEC column for determination of their molar mass average and distribution. Parent homopolymers present in the block copolymer at very low concentrations below 1% of can be traced and characterized, by this novel approach.
Keynote Forum
Rafael Lucena Rodriguez
University of Cordoba, Spain
Keynote: Composites of polyamides and nanoparticles for dispersive micro-solid phase extraction
Time : 10:05-10:30
Biography:
Rafael Lucena Rodríguez is Professor at the Analytical Chemistry Department of the University of Córdoba since 2010. He has co-authored 80 scientific articles and several chapters mainly on microextraction techniques. He has been Guest Editor in one special issue of Analytical and Bioanalytical Chemistry journal devoted to this field. He is the Editor of Microextraction Tech blog. His main research interest comprises different areas, especially the development of new microextraction techniques as well as the evaluation of ionic liquids and nanoparticles in this context. Nowadays, he is also working on biorecognition.
Abstract:
Polymeric materials are widely employed in (micro) extraction techniques due to several advantages such as their stability and versatility. Polyamides, a specific type of polymers, are obtained by the controlled synthesis between a diacid and a diamine compounds yielding a polymeric chain where amide groups are distributed periodically. The chemical forces that allow the chain stacking can also be used for the extraction of target compounds from samples of different nature. In addition, polyamides can be easily synthesized in the lab using several monomers with different moieties that may increase the potential of these polymers as sorbents since the material can be synthesized to boost the interaction with the target compounds. The introduction of nanoparticles inside the polymeric network has been demonstrated as a good way to improve the extraction capacity of the sorbents as well as their mechanical properties. On the one hand, nanoparticles disturb the normal stacking of the polymer increasing their superficial area which results critically to improve both thermodynamics and kinetics aspects of the extraction. On the other hand, the use of special nanoparticles, like magnetic ones, may provide singular properties to the resulting composite. In this communication, the easy synthesis of polyamides-NPs composites, their main advantages and disadvantages will be described in detail to clearly show the potential of this material. This potential, which is supported by practical application of these composites in fields as different as food analysis or bioanalysis, will be explained with suggestions of further research.
- Applications of Seperation Techniques
Location: Melia Meeting
Chair
Morten Lykkegaard Christensen
Aalborg University, Denmark
Co-Chair
Chun Yi Chen
Tokyo Institute of Technology, Japan
Session Introduction
Leon Gradon
Warsaw University of Technology, Poland
Title: Performance of the deep bed filter at its loading with particles and microorganisms
Time : 10:45-11:05
Biography:
Leon GradoÅ„ is a tenured Professor of Chemical Engineering at Warsaw University of Technology. With 45 years of academic and research experience he is author and co-author of 19 monographs and chapters, 4 academic books, over 220 per-reviewed papers in scientific international journals and 65 patents. Several applications of his inventions at technical scale are: Ultrasonic nebulizer, pneumatic nebulizer, bag filters, disposable respirators, dry powder inhaler and diesel engine filters. He is Editorial Board Member in four international journals: Chemical and Process Engineering Journal, International Journal Occupational Safety and Ergonomics, Journal of Aerosol in Medicine, KONA – Powder and Particles, Advanced Powder Technology.
Abstract:
The deep-bed filtration technique is one of the most effective available water purification methods. The real fibrous filter performance emphasizes during its loading, when the deposits gradually change a filtration space. The evolution of temporary filtration efficiency, pressure drop and dust capacity characterizes filter quality. This work is focused on the behavior of filter loading with abiotic and biotic objects presented in the water stream. Multilayer gradient and composite micro- and nanofibrous cartridges were produced for testing using the melt-blown technology. The entire filtration time was divided into 5 periods. After each period of time the tested filter was dried and stratified into layers differing in fiber diameter and porosity. The retention capacity of each layer was calculated gravimetrically. Porosity of the initial fibrous structure and the structure loaded with the deposit collected on the fibers was determined using a scanning electron microscopy in 2-dimentional space. Experimental data presents the time developing values of filtration efficiency and pressure drop for each type of the tested filters. Results show qualitative difference of filters behavior depending on their initial structure. Deposits are distributed inside the cartridge with different manner. When bacteria are present in the water stream, the biofouling due to their intrinsic colonization significantly changes the filter loading. The introduction of nanocomposite Ag and ZnO particle on the filter fiber significantly reduces this effect, according of our measurements.
Morten Lykkegaard Christensen
Aalborg University, Denmark
Title: Impact of sludge properties on solid-liquid separation of activated sludge
Time : 11:05-11:25
Biography:
Morten Lykkegaard Christensen has completed his PhD from Aalborg University. He is the Associated Professor at Aalborg University and Head of the Separation Science Group. He has published more than 40 papers in reputed journals.
Abstract:
Solid-liquid separation of activated sludge is important both during the biological treatment of wastewater and for dewatering of excess sludge. The separation of solid from the treated wastewater can be done by using clarifiers (conventional plants) or membranes (MBR). Further, excess sludge is usually mechanical dewatered before further handling. Solid-liquid separation is a costly part of wastewater treatment. The separation process depends on the composition and the properties of the sludge. Sludge contains sludge flocs, filaments, single cells, dissolved extracellular polymeric substances (EPS) and ions. The best separation is obtained for sludge that contains strong, compact flocs without single cells and EPS. Di- and trivalent ions improve the floc strength and improve the separation whereas monovalent ions (e.g. from road salt, sea water intrusion and industry) impairs the separation. High pH e.g. due to the inlet flow impairs the separation process due to floc disintegration. In membrane operation, single cells and dissolved EPS clogs the membrane whereas strong sludge flocs courses the membrane and thereby reduce membrane fouling. In filtration dewatering small cells and EPS, blinds the cake and thereby lowers the dewaterability. Thus, in all separation processes single cells and dissolved EPS should be avoid e.g. anaerobic storage and high shear levels should be avoid as this erode the flocs and results in more single cells. Sludge can be aerated during storage or nitrate added to avoid anaerobic condition. Further, pumping and mixing should be gently and pipes with sharp bends should be avoided.
Raquel Ibanez Mendizabal
University of Cantabria, Spain
Title: Nanofiltration for the separation of polivalent and monovalent ions in high saline solutions
Time : 11:25-11:45
Biography:
R Ibáñez is Associate Professor of Chemical Engineering at the Chemical and Biomolecular Engineering Department of the University of Cantabria (Spain). She coordinates the research group IPS “Sustainable Processes Engineering” focusing her work in the research and development of advanced separation technologies and their sustainable applications. She has been involved in more than 30 national and international research projects and has been Coordinator of about 10 research projects. She has published more than 60 papers in reputed journals and participates regularly in international conferences
Abstract:
This work, as part of a global membrane process for the recovery of alkali and acids from reverse osmosis (RO) desalination brines, focuses on the nanofiltration separation (NF) of polyvalent and monovalent anions, more specifically sulfate and chloride. This pretreatment stage plays a key role in the whole recovery process. Working with model brines simulating the concentration of RO concentrates, 0.2–1.2 M chloride concentration and 0.1 M sulfate concentration, the experimental performance and modeling of the NF separation is reported. The study has been carried out with the NF270 (Dow Filmtec) membrane. The effect of operating pressure (500–2000 kPa), ionic strength (0.4–1.3 M) and chloride initial concentration (0.2–1.2 M) on the membrane separation capacity has been investigated. Aditionaly, the Donnan steric pore model (DSPM) together with experimentally determined parameters, effective pore radius (rp), thickness of the membrane effective layer (δ) and effective membrane charge density (Xd), was proved accurate enough to satisfactorily describe the experimental results providing the tools for process design and optimization. Finally, In this work, we provide for the first time the analysis of partitioning effects and transport mechanism in the NF separation of sulfate and chloride anions in concentrations that simulate those found in RO desalination brines
Svetlana M. Krylova
York University, Canada
Title: Identification of inhibitors of the Fe-2OG dependent oxygenases by capillary electrophoresis with laser-induced fluorescence
Time : 12:45-13:05
Biography:
S M Krylova obtained her PhD from the Russian Academy of Sciences. She has over ten years of research leadership experience in the area of Medical Diagnostics and Drug Development in biotechnology and pharmaceutical companies in Canada. She has been a Contract Faculty Member at York University in Toronto since 2008. She is also leading research projects in the area of Bioanalytical Chemistry as a Senior Research Associate in the Centre for Research on Biomolecular Interactions at York University.
Abstract:
2-oxoglutarate and Fe (II)-dependent dioxygenases catalyze the removal of N-alkyl groups from damaged DNA in eukaryotes and bacteria. Silencing these demethylating enzymes may be beneficial for the enhancement of chemotherapeutic treatments and reduction of their cytotoxic effects. Therefore, a direct and efficient quantitative analysis using biologically-relevant substrates is needed for detection of demethylase activity of E. coli and human Fe-2OG dioxygenases and its application for high throughput screening of potential inhibitors. Previously reported techniques either utilize coupled enzyme reactions, detect co-products, require complex processing or use radioactive substances. We developed a direct and rapid method based on capillary electrophoresis with laser-induced detection allowing real-time detection of both the substrate and the product separated with high efficiency using no post-enzymatic processing and without the use of radioactive substances. Here we report the CE-based activity assay of two members of the Fe-2OG dioxygenase superfamily of enzymes-hABH2 and hABH3, and demonstrate that the activity can be selectively inhibited by small molecules or short DNA aptamers. The inhibition selectivity of hABH2 over hABH3 enzymes can be advantageously used for qualitative and quantitative assay of these enzymes mixtures. The simple and specific differential analysis can be potentially employed to distinguish hABH2 and hABH3 enzymes expressed by the same types of cells in vivo. The minimal processing, short analysis time, low cost and availability of automation makes this assay useful for developing therapies targeting Fe-2OG dioxygenases.
Conidi Carmela
University of Calabria, Italy
Title: Separation of heath compounds in Goji (Lyceum barbarum) aqueous extracts by membrane technology
Time : 13:05-13:25
Biography:
Conidi Carmela has completed her PhD from University of Calabria and the Institute on Membrane Technology, ITM-CNR. She has done her and Post-doctoral studies from Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de València, Spain. She is a Post-doctoral researcher at the Institute of Membrane Technology of CNR, where she is involved in different research activities devoted to the purification and concentration of antioxidant compounds in products and by-products of food processing. She has published more than 30 papers in international journals.
Abstract:
In recent years, the recovery of antioxidant compounds from natural sources is a focus of great interest due to their potential use as natural ingredients in food, pharmaceutical and cosmetic formulations or as substitutes of synthetic products in the food industry. Several conventional extraction techniques have been reported for the recovery of target compounds from raw materials, such as solvent extraction, ultrasound-assisted extraction, pressurized-liquid extraction, supercritical fluid extraction and resin-based extraction. These extraction methods are characterized by some drawbacks, including the degradation of the target compounds due to high temperatures and long extraction times (as in solvent extractions) and health related risks. Membrane operations are recognized as powerful tools for the purification and concentration of various solutions (e.g., juices, extracts and whey) and the separation of valuable compounds from different food matrix. This study was aimed at developing a sustainable process for the purification of natural antioxidants from Goji berries and leaves. This process is based on the combination of an aqueous extraction with membrane operations in order to avoid the use of organic solvent or adsorbents. The aqueous extraction was studied in order to obtain the maximum yield of phenolic compounds. At this purpose, different parameters such as the extraction time and temperature, the pH and the solid/liquid ratio, were optimized. Aqueous extracts were processed through membrane operations, such as ultrafiltration (UF) and nanofiltration (NF), in order to evaluate the overall bioactivity of fractionated extracts in comparison with that of the unprocessed extracts.
- Emerging separation technologies
Chair
Leon Gradon
Warsaw University of Technology, Poland
Co-Chair
Sandrine Ricote
Colorado School of Mines, USA
Session Introduction
Helene Marie
Dow Benelux BV, Netherland
Title: Understanding and predicting industrial membranes performances in separation technology
Time : 14:10-14:30
Biography:
Hélène Marie has completed her PhD from Compiegne University of Technology (France) and CEA Leti. She is a Lead Engineer at Dow Benelux B.V. in Corporate R&D and works closely with the Dow Water & Process Solution Business Unit.
Abstract:
Nanofiltration (NF) membranes cover a range of salt and neutral molecule selectivities that lay between reverse osmosis and ultrafiltration membranes. This unique performance enabled a breakthrough in industry during the past decades. Their high water permeability along with good rejection makes them usable as a replacement for different treatment processes at a reasonable energy cost. However, there are some hindrances in the design of new NF processes and improvement of existing ones since NF applications are mostly associated with complex transport phenomena of which modeling is difficult. The understanding of transport phenomena through NF membranes by use of modeling and prediction may help to implement NF membrane technology more broadly and will enlarge the NF market beyond water filtration. Considering that NF is an industrial scale method for purification and concentration of oligosaccharides mixtures, we decided to study some industrial membranes performances against various saccharides. We studied independently each component of the solute/membrane/solvent system and their pair-interaction to achieve a deep theoretical knowledge. We then collected rejection data and analyzed membrane performances while varying parameters, such as temperature and concentration. Thanks to the characterization of the membranes and their performance, we were able to parameterize a model based on the extended Nernst-Planck equation for rejection results. Finally, the model was assessed against mixture separations. Going forward, the outcome of this study will enable more rigorous membrane selection for targeted industrial applications and to predict its performance.
Kai Wilkner
Forschungszentrum Julich, Germany
Title: Influence of coal power plant exhaust gas on the structure and performance of ceramic nanostructured gas separation membranes
Time : 14:30-14:50
Biography:
K Wilkner holds a Diploma in Physical Engineering from the University of Applied Science Aachen. Since 2011, he has been working at the Institute of Energy and Climate Research: Materials synthesis and processing (IEK-1) in Forschungszentrum Jülich. Since 2012, one of his responsibilities is to test membranes in direct contact with flue gas of lignite- and hard-coal-fueled power plants.
Abstract:
Carbon capture and storage or utilization is a key technology to decrease CO2 emissions from conventional power plants, until cost efficient energy supply from renewable sources is possible. Membrane-based systems to capture CO2 from flue gas streams are considered a promising alternative to conventional absorption technology. In the present work the effect of coal power plant exhaust gas on amino-modified mesoporous ceramic membranes was investigated. Testing membranes in direct contact with exhaust gas represents a new approach, as testing under simulated flue gas conditions has already been undertaken. Flue gas exposure experiments were carried out at a lignite-fueled power plant and a hard-coal-fueled power plant. Most experiments were conducted using a test rig designed to bring planar membranes samples in direct contact with unconditioned flue gas in the exhaust gas channel. Another test rig was designed to test membrane modules with pre-treated flue gas. The tested membranes had an asymmetric structure consisting of a macroporous α-Al2O3 support coated with a mesoporous É£-Al2O3 or 8YSZ interlayer. The microporous functional top layer was made of amino-functionalized silica. The tests revealed different degradation mechanisms such as gypsum/fly ash deposition on the membrane surface, pore blocking by water condensation, chemical reactions and phase transformation. A detailed analysis was carried out by XRD, XPS and SEM to evaluate their impact on the membrane in order to assess membrane stability under real conditions. The suitability of these membranes for this application is critically discussed and an improved mode of membrane operation is proposed.
Feng Zhang
Chinese Academy of Inspection & Quarantine, China
Title: A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and metabolites in serum samples by ultra-high performance supercritical fluid chromatography
Time : 14:50-15:10
Biography:
Feng Zhang has completed his PhD from Dalian Institute of Chemical Physics, Chinese Academy of Sciences in the year 2005. He has done his Post-doctoral research from Max Planck Institute of Biochemistry under the funding of Max-Planck Society. He has worked as a Senior Expert of Food Safety at the Chinese Academy of Inspection and Quarantine. In 2014, he was assigned as the Director of the Institute of Food Safety and focusing on Chromatography/Mass Spectrometry Techniques applied to food, tobacco, proteomics, metabolomics and pharmaceutical research. He has published more than 40 papers in reputed journals, 4 books and has authorized 3 patents.
Abstract:
Sulfonamides are a large group of synthetic antibiotics which has anti-bacterial properties. They have a good antibacterial effect on both Gram-positive and Gram-negative bacteria. Sulfonamides are commonly prescribed in human and veterinary medicine against many kinds of infections. In order to investigate the pharmacodynamics and pharmacokinetics of different sulfonamides, establishing a detection method of multiple sulfonamides and their metabolites in serum is necessary. An analytical method based on ultra-high performance supercritical fluid chromatography (UHPSFC) with photo-diode array detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Under the optimized gradient elution conditions, it took only 7 min to separate all 15 sulfonamides and the critical pairs of each parent drug and metabolite were completely separated. Variables affecting the UHPSFC were optimized to get a better separation. The performance of the developed method was evaluated. The UHPSFC method allowed the baseline separation and determination of 15 sulfonamides and metabolites with a limit of detection ranging from 0.15 to 0.35 g/mL. Recoveries between 90.1 and 102.2% were obtained with satisfactory precision since relative standard deviations were always below 3%. Hence, the proposed method is simple, accurate, time-saving and green; it is applicable to a variety of sulfonamides detection in serum samples.
Sandrine Ricote
Colorado School of Mines, USA
Title: Protonic ceramic membranes under asymmetric steam atmosphere
Time : 15:10-15:30
Biography:
Sandrine Ricote obtained her PhD on Ceramic Proton Conductors at the University of Burgundy, France. She worked four years in the Department of Energy Conversion and Storage at the Danish Technical University as a Post-doc and then as a Scientist. In 2012, she moved to the Department of Mechanical Engineering at Colorado School of Mines. She is now a Research Associate Professor, with a main focus on ceramic proton conductors.
Abstract:
A comprehensive analysis of proton transport in protonic ceramic membrane devices is presented. Thin, dense membranes of BaZr0.8Ce0.1Y0.1O3-d, BZCY81, may now be fabricated with relative ease at commercial scale. These devices have potential for supporting the emerging hydrogen economy and reducing dependence of fossil fuels. With protonic ceramic electrolytes it is possible to galvanically transport pure hydrogen from one side of a membrane to the other, making it possible to fabricate electrochemical devices and systems that were previously impractical or impossible. H2 can be produced from natural gas by steam reforming, whereby hydrogen may be extracted from a reacting stream of methane and steam in a protonic membrane reformer, PMR; Liquid hydrocarbons, such as ethylene and benzene, may be produced from dry methane in a catalytic membrane reactor by methane dehydroaromatization, MDA, with hydrogen extracted from the feed gas; Ammonia can be synthesized by pumping hydrogen through the membrane to react with nitrogen in a process called solid-state ammonia synthesis, SSAS; and H2 can be produced from water vapor by steam electrolysis in a protonic ceramic electrolysis cell (PCEC). In order for these devices to become commercially viable, a clear understanding of their operation in various use environments is necessary. All the devices listed above consume electric power to pump hydrogen across the membrane, which must be supplied by an external power source. The power consumed is the product of the applied voltage and the current consumed by the galvanic device, so it is important that the device have low resistance and high faradaic efficiency with respect to proton transport and that power is not wasted by parasitic losses. The proton current depends on the effective resistance, which depends on electrode performance, faradaic efficiency and bulk materials properties. Well-designed electrodes can, in principle, be developed with low effective resistance, but in the final analysis it is the conductivity of the electrolyte membrane that limits the performance of these devices. The proton conductivity of BZCY81, is only a few millisiemens per centimeter in reducing atmosphere. More importantly, conductivity in BZCY is a strong function of water vapor pressure, making the electrolyte a mixed proton/steam conductor. This is an unusual characteristic that is unique to protonic ceramic electrolytes. The impact depends on the application. For example, MDA requires nominally dry methane on the feed side, while PMR requires moist atmosphere with steam-to-carbon ratio greater than unity. SSAS, on the other hand requires dry atmosphere on the permeate side, where nitrogen reacts with hydrogen to produce anhydrous ammonia. Steam electrolysis is carried out under moist oxidizing conditions on the feed side. In all cases, the desired permeate is hydrogen, as dry as possible to avoid the need for separation of hydrogen from steam, but as a practical matter, some steam will be present either due to steam permeation or added to the sweep gas intentionally. The transport properties of the membrane in each of these devices depend strongly on pH2O and pH2 on the feed and permeate sides. Knowledge of the electrolyte conductivity as a function of pH2O and pH2 on each side of the membrane is essential for designing cost-effective galvanic systems since this determines the protonic flux density.
Robert van Ling
Thermo Fisher Scientific, Netherlands
Title: Separation science that's built for biopharma
Time : 15:45-16:05
Biography:
Robert van Ling has started working at LC Packings, focusing on Nano and Capillary LC. Following the successful introductions of UltiMate, the world’s first Nano LC system, LC Packings was acquired by Dionex Corp. in which he has held several roles in conveying the use of Nano LC-MS in Proteomics, for both ESI and MALDI applications, and later moving more towards separation of intact proteins and Protein Therapeutics. Since 2012, he has been working as a Member of the Thermo Fisher Scientific family. Currently, he is supporting wide portfolio of Chromatography Consumables and specifically sample preparation and characterization of large molecules and biologics.
Abstract:
Biopharmaceuticals now dominate the leading positions in both sales and revenue tables over traditional small molecule drugs and drug pipelines continue to lead by biopharmaceuticals. In addition, as some of the earlier biopharmaceuticals begin to come off patent, we are seeing tremendous growth and attention in biosimilars. Both biopharmaceuticals and biosimilars however present new challenges compared to small molecules due to their complexity and variability; and as such manufacturers need advanced analytical tools to be able to effectively characterize these biopharmaceuticals to ensure efficacy and safety of the product. In this seminar, I will overview the latest advances made by Thermo Fisher Scientific with respect to UHPLC instrumentation and column chemistries for complete biopharmaceutical separation and characterization. The Thermo Scientific™ Vanquish™ Flex UHPLC system is truly built for biopharma offering precise and reproducible separations, coupled with flexibility to execute all major biopharmaceutical applications. Our extensive portfolio of column chemistries combines with the Vanquish Flex to offer a complete solution for biopharmaceutical analysis. Examples of characterizing monoclonal antibodies to determine amino acid sequence, charge variants, aggregates, purity and glycan profiles will be presented.
Snežana Maletic
University of Novi Sad, Republic of Serbia
Title: Removal of divalent heavy-metal ions from aqueous solutions by adsorption process with titanium dioxide nanowires
Time : 16:05-16:25
Biography:
Snežana Maletić is Associate Professor at the chair of Chemical Technology and Environmental Protection, University of Novi Sad Faculty of Sciences. Her education qualifications include: PhD in Chemistry from University of Novi Sad Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection in 2010; BSc in Chemistry from University of Novi Sad Faculty of Sciences, Department of Chemistry in 2003. Her research interests include: Environmental protection, chemical technology, remediation of contaminated water, soil and sediment, bioavailability of inorganic/organic pollutants in sediments/soils investigation, adsorption of inorganic pollutants on nanomaterials. She is Head of the Laboratory for the analysis of environmental samples accredited according to ISO 17025 protocols.
Abstract:
The key objective of this work was to investigate kinetics and adsorption capacities of divalent metal ions (Cu2+, Pb2+, Cd2+) from water on TiO2 nanowires at pH 3 and 7. Brunauer-Emmett-Teller (BET) analysis showed that the surface area of the TiO2 nanowires was 115,9 m2g-1. The point of zero charge (pHpzc) was 4.8. Adsorption experiments were performed using the conventional batch technique at room temperature (25±2°C). The background solution was 0.01 M CaCl2 in deionised water. Initial concentrations of heavy metal ions were in the range 0.05–5 mg L-1. The amount of adsorbent corresponded to a sample/solution ratio that resulted in 20-80% uptake of given metal ion. The samples were agitated on horizontal shaker for 30 h. The time to reach adsorption equilibrium was obtained from a kinetic study performed over 72 h. The adsorption kinetics of divalent metal ions on TiO2 nanowires was investigated using pseudo-first order, pseudo-second order and intraparticle diffusion models. Adsorption of metal ions was controlled by chemisorption which was supported by the suitability of the pseudo-second order model. Intraparticle diffusion model data showed that diffusion cannot be considered as the limiting step of adsorption. The equilibrium adsorption data were modeled using Freundlich and Langmuir adsorption isotherms which both showed good agreement with experimental data. The highest removal efficiency of heavy metals was observed at pH=7, except in the case of Pb which has been removed better at pH=3. Adsorption affinities increased in the following order: PbpH3> CdpH7 >PbpH7 > CdpH3> CupH7 > CupH3.
Mohamad Houssam Al balkhi
Universite de Picardie Jules Verne, France
Title: Development of a liquid-liquid extraction method of resveratrol from cell culture media using solubility parameters
Time : 16:25-16:45
Biography:
Mohamad Houssam Al balkhi has obtained his PhD in 2009 from the University of Picardie Jules Verne (France). He worked as assistant professor at the University of Damascus (Syria) for many years. Actually, he is a post-doctoral fellow at the University of Picardie Jules Verne working on Plant Biotechnology and the development of extraction methods of active compounds. He has published many papers in reputed journals.
Abstract:
The extraction of bioactive compounds, produced by plant cell cultures, directly from their culture medium, which contains other by-products, is a great challenge. Resveratrol extraction from its grapevine cell cultures is considered here as an example to improve the extraction processes from plant cell cultures using solubility parameters. Successive liquid-liquid extraction (LLE) processes were exploited to extract resveratrol from the culture medium with an extraction ratio approaching 100%, high selectivity and minimum amounts of solvents. The calculations of partition coefficients as a function of solubility parameters demonstrated that benzyl benzoate is the most suitable intermediate solvent to extract resveratrol from its aqueous medium at a benzyl benzoate/medium ratio of 1:100 v/v. The calculations also illustrated the high ability of methanol and ethanol to extract resveratrol from benzyl benzoate. The physicochemical properties of benzyl benzoate and processing conditions were exploited to separate it from aqueous media and organic solvents. The agitation method, component ratios and extraction time were studied to maximize the extraction yield. Under the best studied conditions, the recovery of resveratrol from different culture media approached approximately 100% with a selectivity of approximately 92%. Ultimately, the improved extraction processes of resveratrol are markedly efficient, selective, rapid and economical.
Qu Yuanyuan
Shandong University, China
Title: Highly selective sieving in porous graphene-like carbon nitride for helium/light isotopes separation
Time : 16:45-17:05
Biography:
Mrs. Yuanyuan Qu is researcher from Shandong university, China
Abstract:
An efficient membrane for helium separation from natural gas is quite crucial for cryogenic industries. However, most experimentally available membranes fail in separating helium from small molecules in natural gas, such as H2, as well as in 3He/4He isotopes separation. Using first-principles calculations, we theoretically demonstrated that the already-synthesized graphitic carbon nitride (g-C3N4) has high efficiency in helium separation from the gas molecules (H2, N2, CO and CH4) in natural gas and the noble gas molecules (Ne and Ar). The selectivity of He over H2 molecule at room temperature is calculated to be as high as 107. More interestingly, the g-C3N4 membrane can also serve as a quantum sieving membrane for 3He/4He separation with a predicted transmission ratio of 18 at 49 K, thus offers a combined means of both He and 3He isotope separation. Furthermore, for another experimentally available porous graphene-like carbon nitride (C2N-h2D), we theoretically demonstrated that highly efficient light isotopes separation, such as 3He/4He, can be reached via quantum sieving effect. Under moderate tensile strain, the quantum sieving of the C2N-h2D membrane can be effectively tuned in a continuous way, leading to a temperature window with high 3He/4He selectivity and permeance acceptable for efficient isotopes harvest in industrial application. This mechanism also holds for separation of other light isotopes, such as H2/D2, H2/T2. Such tunable quantum sieving opens a promising avenue for light isotopes separation for industrial application.
Yuyoung Shin
University of Manchester, UK
Title: 2D crystal-based membranes for photocatalysis and separation
Time : 17:05-17:25
Biography:
Yuyoung Shin obtained her BSc in Chemistry from University of Sussex in 2010 and her MPhil in Chemistry from University of Cambridge in 2012, working with Dr Mark Miller on thermodynamics of charged nano-droplets. Since 2014, funded by EPSRC, she undertook her PhD with Prof. Cinzia Casiraghi at University of Manchester, working on synthesis and characterization of graphene-based membranes. During her studies, she contributed to a number of publications, and recently had two publications on synthesis and characterization of graphene-based membranes.
Abstract:
The isolation of graphene has unveiled a wide range of novel 2-Dimensional (2D) materials with outstanding properties. Liquid-phase exfoliation (LPE) is a simple technique for production of 2D-crystal dispersions, which can be used to form coatings and membranes. 2D crystal-based membranes have already shown interesting properties, such as selective permeation of water, opening the possibility of using these membranes for gas or liquid separation. In this talk, we give two examples of 2D crystal-based membranes. The first membrane is obtained by LPE of graphitic carbon nitride (g-C3N4), which has been shown to be an efficient photo-catalyst for many reactions under visible light. Photo-degradation studies show that the membranes are very efficient in the degradation of several dyes. This is attributed to the membrane structure: As the catalyst is a porous laminate, the reactant can flow through the pores of the membrane and because the space between the g-C3N4 nanosheets is comparable to the size of the dyes, the probability of the reactants to be close to the catalyst is enhanced, making the reaction very efficient. The second type of 2D-crystal membrane is prepared by mixing LPE graphene with a polymer of intrinsic microporosity (PIM-1). Graphene is expected to improve membrane permeability, control over diffusion selectivity and to reduce the polymer ageing. Here we show characterization of those membranes by Raman spectroscopy and transmission electron microscopy and we show preliminary results on CO2 permeability.